Host-Pathogen Interactions of Actinobacillus pleuropneumoniae with Porcine Lung and Tracheal Epithelial Cells

被引:85
作者
Auger, Eliane [1 ,2 ]
Deslandes, Vincent [1 ,2 ]
Ramjeet, Mahendrasingh [1 ,2 ]
Contreras, Irazu [3 ]
Nash, John H. E. [4 ]
Harel, Josee [1 ,2 ]
Gottschalk, Marcelo [1 ,2 ]
Olivier, Martin [3 ]
Jacques, Mario [1 ,2 ]
机构
[1] Univ Montreal, Fac Med Vet, Grp Rech Malad Infect Porc, St Hyacinthe, PQ J2S 7C6, Canada
[2] Univ Montreal, Fac Med Vet, Ctr Rech Infectiol Porcine, St Hyacinthe, PQ J2S 7C6, Canada
[3] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada
[4] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
NF-KAPPA-B; LIPOPOLYSACCHARIDE OUTER CORE; RESPIRATORY-TRACT CELLS; ESCHERICHIA-COLI; HAEMOPHILUS-INFLUENZAE; TRANSCRIBED SEQUENCES; SELECTIVE CAPTURE; SURFACE FIBRILS; NATURAL HOST; RTX TOXINS;
D O I
10.1128/IAI.00297-08
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Host-pathogen interactions are of great importance in understanding the pathogenesis of infectious microorganisms. We developed in vitro models to study the host-pathogen interactions of porcine respiratory tract pathogens using two immortalized epithelial cell lines, namely, the newborn pig trachea (NPTr) and St. Jude porcine lung (SJPL) cell lines. We first studied the interactions of Actinobacillus pleuropneumoniae, an important swine pathogen, using these models. Under conditions where cytotoxicity was absent or low, we showed that A. pleuropneumoniae adheres to both cell lines, stimulating the induction of NF-kappa B. The NPTr cells consequently secrete interleukin 8, while the SJPL cells do not, since they are deprived of the NF-kappa B p65 subunit. Cell death ultimately occurs by necrosis, not apoptosis. The transcriptomic profile of A. pleuropneumoniae was determined after contact with the porcine lung epithelial cells by using DNA microarrays. Genes such as tadB and rcpA, members of a putative adhesin locus, and a gene whose product has high homology to the Hsf autotransporter adhesin of Haemophilus influenzae were upregulated, as were the genes pgaBC, involved in biofilm biosynthesis, while capsular polysaccharide-associated genes were downregulated. The in vitro models also proved to be efficient with other swine pathogens, such as Actinobacillus suis, Haemophilus parasuis, and Pasteurella multocida. Our results demonstrate that interactions of A. pleuropneumoniae with host epithelial cells seem to involve complex cross talk which results in regulation of various bacterial genes, including some coding for putative adhesins. Furthermore, our data demonstrate the potential of these in vitro models in studying the host-pathogen interactions of other porcine respiratory tract pathogens.
引用
收藏
页码:1426 / 1441
页数:16
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