TRIM44 interacts with and stabilizes terf, a TRIM ubiquitin E3 ligase

被引：58

作者：

Urano, Tomohiko ^{[1
,2
,3
]}

Usui, Takahiko ^{[1
,2
]}

Takeda, Shizu ^{[4
]}

Ikeda, Kazuhiro ^{[3
]}

Okada, Atsushi ^{[1
,2
]}

Ishida, Yoshiko ^{[4
]}

Iwayanagi, Takao ^{[4
,5
]}

Otomo, Jun ^{[4
]}

Ouchi, Yasuyoshi ^{[1
]}

Inoue, Satoshi ^{[1
,2
,3
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Geriatr Med, Bunkyo Ku, Tokyo 1138655, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Antiaging Med, Bunkyo Ku, Tokyo 1138655, Japan

[3] Saitama Med Sch, Res Ctr Genom Med, Hidaka, Saitama 3501241, Japan

[4] Hitachi Ltd, Cent Res Lab, Kokubunji, Tokyo 1858601, Japan

[5] Natl Inst Genet, Lab Res & Dev Biol Database, Mishima, Shizuoka 4118540, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 383卷 / 02期

关键词：

TRIM44; Terf; RING finger; E3 ubiquitin ligase; LINDAU TUMOR-SUPPRESSOR; PROTEIN; IDENTIFICATION; DEGRADATION; DEUBIQUITINATION; MECHANISMS; MID1; RO52;

D O I：

10.1016/j.bbrc.2009.04.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Terf/TRIM17 is a member of the TRIM family of proteins, which is characterized by the RING finger, B-box, and coiled-coil domains. In the present Study, we found that terf interacts with TRIM44. Terf underwent ubiquitination in vitro in the presence of the E2 enzyme UbcH6; this Suggests that terf exhibits E3 ubiquitin ligase activity. It was also found that terf was conjugated with polyubiquitin chains and stabilized by the proteasome inhibitor in mammalian cells: this Suggested that terf tendered itself susceptible to proteasomal degradation through polyubiquitination. We also found that TRIM44 inhibited ubiquitination of terf, and thus stabilized the protein. The N-terminal region of TRIM44 contains a zinc-finger domain found in ubiquitin hydrolases (ZF UBP) and ubiquitin specific proteases (USPs). Thus, we proposed that TRIM44 may function as a new class of the "USP-like-TRIM" which regulates the activity of associated TRIM proteins. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：263 / 268

页数：6

共 22 条

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