In vitro investigation of toxicological interactions between the fusariotoxins deoxynivalenol and zearalenone

被引：43
作者：
Bensassi, Fatma [1 ,2 ]
Gallerne, Cindy [3 ]
el Dein, Ossama Sharaf [3 ]
Hajlaoui, Mohamed Rabeh [2 ]
Lemaire, Christophe [3 ,4 ]
Bacha, Hassen [1 ]
机构：
[1] Fac Dent, Lab Res Biol Compatible Cpds, Monastir 5019, Tunisia
[2] INRA Tunisia, Natl Inst Agr Res, Lab Plant Protect, Ariana 2049, Tunisia
[3] INSERM, U769, LabEx LERMIT, Fac Pharm, Chatenay Malabry, France
[4] Univ Versailles St Quentin, F-78035 Versailles, France
来源：
TOXICON | 2014年 / 84卷
关键词：
Deoxynivalenol; Zearalenone; Combination; Apoptosis; Mitochondria; PTP; STATISTICALLY DESIGNED EXPERIMENTS; OXIDATIVE STRESS; MEMBRANE PERMEABILIZATION; CHROMOSOME-ABERRATIONS; FUSARIUM MYCOTOXINS; NATURAL OCCURRENCE; T-2; TOXIN; CELLS; TRICHOTHECENES; APOPTOSIS;
D O I：
10.1016/j.toxicon.2014.03.005
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
It is expected that humans are exposed to combined mycotoxins, which occur simultaneously in the food items, than to individual compounds and that can increase their potential toxicity. Considering this coincident production, deoxynivalenol (DON) and zearalenone (ZEN) as they are produced by several Fusarium species, can interfere at a cellular level. Therefore, these two toxins were chosen to study their interactive effects on human colon carcinoma cells (HCT116), using the endpoints including cell viability, cell cycle analysis, mitochondrial transmembrane potential (Delta psi m) determination and permeability transition pore (PTP) opening. Our results showed that DON and ZEN caused a marked decrease of cell viability in a dose-dependent manner, mediated by an activation of the mitochondrial apoptotic process; characterized by PTP opening and the loss of Delta psi m. Nevertheless, combined DON and ZEN reduced all the toxicities observed with the mycotoxins separately. Therefore, the combination of the two mycotoxins appears as a subadditive response. (C) 2014 Elsevier Ltd. All rights reserved.
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页码：1 / 6
页数：6
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