Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae

被引：86
|
作者：
Palmieri, L
Lasorsa, FM
Iacobazzi, V
Runswick, MJ
Palmieri, F
Walker, JE
机构：
[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 2DH, England
[2] Univ Bari, Dept Pharmacobiol, Biochem & Mol Biol Lab, I-70125 Bari, Italy
来源：
FEBS LETTERS | 1999年 / 462卷 / 03期
关键词：
yeast; mitochondrion; transport; CRC1; gene; carnitine carrier; YOR100c;
D O I：
10.1016/S0014-5793(99)01555-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mitochondrial carrier protein for carnitine has been identified in Saccharomyces cerevisiae. It is encoded by the gene CRC1 and is a member of the family of mitochondrial transport proteins, The protein has been over-expressed with a C-terminal His-tag in S. cerevisiae and isolated from mitochondria by nickel affinity chromatography, The purified protein has been reconstituted into proteoliposomes and its transport characteristics established. It transports carnitine, acetylcarnitine, propionylcarnitine and to a much lower extent medium- and long- chain acylcarnitines, (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：472 / 476
页数：5
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