Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment

被引：212

作者：

Rodin, Sergey ^{[1
]}

Antonsson, Liselotte ^{[2
,3
]}

Niaudet, Colin ^{[4
]}

Simonson, Oscar E. ^{[5
,6
]}

Salmela, Elina ^{[7
,8
]}

Hansson, Emil M. ^{[9
]}

Domogatskaya, Anna ^{[1
]}

Xiao, Zhijie ^{[1
]}

Damdimopoulou, Pauliina ^{[2
,3
]}

Sheikhi, Mona ^{[2
,3
]}

Inzunza, Jose ^{[2
,3
]}

Nilsson, Ann-Sofie ^{[1
]}

Baker, Duncan ^{[10
]}

Kuiper, Raoul ^{[11
]}

Sun, Yi ^{[12
]}

Blennow, Elisabeth ^{[13
,14
]}

Nordenskjold, Magnus ^{[13
,14
]}

Grinnemo, Karl-Henrik ^{[5
,6
]}

Kere, Juha ^{[7
,8
,15
,16
]}

Betsholtz, Christer ^{[4
]}

Hovatta, Outi ^{[2
,3
]}

Tryggvason, Karl ^{[1
,17
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, SE-17177 Stockholm, Sweden

[2] Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Obstet & Gynecol, SE-14186 Huddinge, Sweden

[3] Karolinska Univ Hosp, SE-14186 Huddinge, Sweden

[4] Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden

[5] Karolinska Inst, Adv Ctr Translat Regenerat Med ACTREM, Dept Mol Med & Surg, Div Cardiothorac Surg & Anaesthesiol, SE-17177 Stockholm, Sweden

[6] Karolinska Univ Hosp, SE-17177 Stockholm, Sweden

[7] Univ Helsinki, Res Programs Unit, FI-00014 Helsinki, Finland

[8] Folkhalsan Inst Genet, FI-00014 Helsinki, Finland

[9] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA

[10] Sheffield Childrens NHS Trust, Sheffield Diagnost Genet Serv, Sheffield S10 2TH, S Yorkshire, England

[11] Karolinska Hosp, Karolinska Inst, FENO, Dept Lab Med, SE-14186 Stockholm, Sweden

[12] BioLamina AB, S-17266 Sundbyberg, Sweden

[13] Karolinska Univ Hosp, Dept Mol Med & Surg, S-17176 Stockholm, Sweden

[14] Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, S-17176 Stockholm, Sweden

[15] Karolinska Inst, Dept Biosci & Nutr, SE-14183 Stockholm, Sweden

[16] Karolinska Inst, Sci Life Lab, SE-14183 Stockholm, Sweden

[17] Duke NUS, Cardiovasc & Metab Disorders Program, Singapore 169857, Singapore

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

基金：

英国医学研究理事会; 瑞典研究理事会;

关键词：

TERM SELF-RENEWAL; E-CADHERIN; LINES; GROWTH; DERIVATION; EXPRESSION; EXPANSION; APOPTOSIS; SURVIVAL; ADHESION;

D O I：

10.1038/ncomms4195

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes.

引用

页数：13

共 44 条

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[44] Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells
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