Interplay between Glass Formation and Liquid-Liquid Phase Separation Revealed by the Scattering Invariant

被引：18

作者：

Da Vela, Stefano ^{[1
]}

Begam, Nafisa ^{[1
]}

Dyachok, Danylo ^{[1
]}

Schaeufele, Richard Santiago ^{[1
]}

Matsarskaia, Olga ^{[1
]}

Braun, Michal K. ^{[1
]}

Girelli, Anita ^{[1
]}

Ragulskaya, Anastasia ^{[1
]}

Mariani, Alessandro ^{[2
]}

Zhang, Fajun ^{[1
]}

Schreiber, Frank ^{[1
]}

机构：

[1] Univ Tubingen, Inst Angew Phys, D-72076 Tubingen, Germany

[2] European Synchrotron Radiat Facil, F-38043 Grenoble, France

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2020年 / 11卷 / 17期

关键词：

SPINODAL DECOMPOSITION; SMALL-ANGLE; BEHAVIOR; GELATION; MIXTURE; USAXS;

D O I：

10.1021/acs.jpclett.0c02110

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interplay of the glass transition with liquid-liquid phase separation (LLPS) is a subject of intense debate. We use the scattering invariant Q to probe how approaching the glass transition affects the shape of LLPS boundaries in the temperature/volume fraction plane. Two protein systems featuring kinetic arrest with a lower and an upper critical solution temperature phase behavior, respectively, are studied varying the quench depth. Using Q we noninvasively identify system-dependent differences for the effect of glass formation on the LLPS boundary. The glassy dense phase appears to enter the coexistence region for the albumin-YCl3 system, whereas it follows the equilibrium binodal for the gamma-globulin-PEG system.

引用

页码：7273 / 7278

页数：6

共 47 条

[1]

[Anonymous], 1987, Structure Analysis by Small-Angle X-Ray and Neutron Scattering, DOI DOI 10.1007/978-1-4757-6624-0

[2] Physical principles of intracellular organization via active and passive phase transitions [J].

Berry, Joel ;

Brangwynne, Clifford P. ;

Haataja, Mikko .

REPORTS ON PROGRESS IN PHYSICS, 2018, 80 (04)

[3] Spinodal decomposition in a food colloid-biopolymer mixture: evidence for a linear regime [J].

Bhat, Suresh ;

Tuinier, Remco ;

Schurtenberger, Peter .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2006, 18 (26) :L339-L346

[4] Soft-Matter Approaches for Controlling Food Protein Interactions and Assembly [J].

Boire, Adeline ;

Renard, Denis ;

Bouchoux, Antoine ;

Pezennec, Stephane ;

Croguennec, Thomas ;

Lechevalier, Valerie ;

Le Floch-Fouere, Cecile ;

Bouhallab, Said ;

Menut, Paul .

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 10, 2019, 10 :521-539

[5] Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins [J].

Bucciarelli, Saskia ;

Casal-Dujat, Lucia ;

De Michele, Cristiano ;

Sciortino, Francesco ;

Dhont, Jan ;

Bergenholtz, Johan ;

Farago, Bela ;

Schurtenberger, Peter ;

Stradner, Anna .

JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2015, 6 (22) :4470-4474

[6] PHASE SEPARATION BY SPINODAL DECOMPOSITION IN ISOTROPIC SYSTEMS [J].

CAHN, JW .

JOURNAL OF CHEMICAL PHYSICS, 1965, 42 (01) :93-+

[7] Dynamical arrest in attractive colloids: The effect of long-range repulsion [J].

Campbell, AI ;

Anderson, VJ ;

van Duijneveldt, JS ;

Bartlett, P .

PHYSICAL REVIEW LETTERS, 2005, 94 (20)

[8] Interplay between spinodal decomposition and glass formation in proteins exhibiting short-range attractions [J].

Cardinaux, Frederic ;

Gibaud, Thomas ;

Stradner, Anna ;

Schurtenberger, Peter .

PHYSICAL REVIEW LETTERS, 2007, 99 (11)

[9] Arrested and temporarily arrested states in a protein-polymer mixture studied by USAXS and VSANS [J].

Da Vela, Stefano ;

Exner, Christian ;

Schaufele, Richard Santiago ;

Moller, Johannes ;

Fu, Zhendong ;

Zhang, Fajun ;

Schreiber, Frank .

SOFT MATTER, 2017, 13 (46) :8756-8765

[10] Effective Interactions and Colloidal Stability of Bovine γ-Globulin in Solution [J].

Da Vela, Stefano ;

Roosen-Runge, Felix ;

Skoda, Maximilian W. A. ;

Jacobs, Robert M. J. ;

Seydel, Tilo ;

Frielinghaus, Henrich ;

Sztucki, Michael ;

Schweins, Ralf ;

Zhang, Fajun ;

Schreiber, Frank .

JOURNAL OF PHYSICAL CHEMISTRY B, 2017, 121 (23) :5759-5769

← 1 2 3 4 5 →