Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

被引：1
作者：
Zhang, Shuo [1 ]
Erckens, Roel J. [1 ]
Jongsma, Frans H. M. [1 ]
De Brabander, John [1 ]
Webers, Carroll A. B. [1 ]
Berendschot, Tos T. J. M. [1 ]
机构：
[1] Univ Eye Clin Maastricht, Maastricht, Netherlands
来源：
OPTICAL ENGINEERING | 2020年 / 59卷 / 09期
关键词：
Raman spectroscopy; eye; aqueous humor; confocal microscopy; dark-field illumination; TISSUES; GLUCOSE;
D O I：
10.1117/1.OE.59.9.092002
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)
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页数：9
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