Dental Pulp Stem Cells (DPSCs) differentiation study by Confocal Raman Microscopy

被引：0
作者：
Salehi, H. [1 ]
Collart-Dutilleul, P-Y [1 ]
Gergely, C. [2 ,3 ]
Cuisinier, F. J. G. [1 ]
机构：
[1] Univ Montpellier I, UFR Odontol, EA 4203, Lab Biol Sante Nanosci, F-34193 Montpellier, France
[2] Univ Montpellier 2, Lab Charles Coulomb, UMR 5221, F-34095 Montpellier, France
[3] CNRS, Lab Charles Coulomb, UMR 5221, Montpellier, France
来源：
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XII | 2014年 / 8947卷
关键词：
Dental Pulp Stem cells DPSC; Raman microscopy; differentiation; living cell; SPECTROSCOPY;
D O I：
10.1117/12.2041346
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free, real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm(-1) region (C-H stretching) and 960 cm(-1) peak (phosphate PO43-) were collected. In Dental Pulp Stem Cells 21st day differentiated in buffer solution, phosphate peaks nu(1) PO43- (first vibrational mode) at 960cm(-1) and nu(2) PO43- at 430cm(-1) and nu(4) PO43- at 585cm(-1) are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.
引用
收藏
页数：10
相关论文
共 16 条
[1]   The Therapeutic Applications of Multipotential Mesenchymal/Stromal Stem Cells in Skeletal Tissue Repair [J].
Arthur, Agnieszka ;
Zannettino, Andrew ;
Gronthos, Stan .
JOURNAL OF CELLULAR PHYSIOLOGY, 2009, 218 (02) :237-245
[2]   In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts [J].
Chiang, Huihua Kenny ;
Peng, Feng-Ying ;
Hung, Shih-Chieh ;
Feng, Ya-Ching .
JOURNAL OF RAMAN SPECTROSCOPY, 2009, 40 (05) :546-549
[3]  
Coppe C, 2009, PEDIATR DENT, V31, P467
[4]   Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation [J].
d'Aquino, R. ;
Graziano, A. ;
Sampaolesi, M. ;
Laino, G. ;
Pirozzi, G. ;
De Rosa, A. ;
Papaccio, G. .
CELL DEATH AND DIFFERENTIATION, 2007, 14 (06) :1162-1171
[5]   Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].
Dominici, M. ;
Le Blanc, K. ;
Mueller, I. ;
Slaper-Cortenbach, I. ;
Marini, F. C. ;
Krause, D. S. ;
Deans, R. J. ;
Keating, A. ;
Prockop, D. J. ;
Horwitz, E. M. .
CYTOTHERAPY, 2006, 8 (04) :315-317
[6]   Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].
Gronthos, S ;
Mankani, M ;
Brahim, J ;
Robey, PG ;
Shi, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630
[7]  
Hartmann K., 2012, ANAL BIOANAL CHEM
[8]   Near-infrared Raman spectroscopy for optical diagnosis of lung cancer [J].
Huang, ZW ;
McWilliams, A ;
Lui, H ;
McLean, DI ;
Lam, S ;
Zeng, HS .
INTERNATIONAL JOURNAL OF CANCER, 2003, 107 (06) :1047-1052
[9]   Human dental follicle cells acquire cementoblast features under stimulation by BMP-2/-7 and enamel matrix derivatives (EMD) in vitro [J].
Kemoun, Philippe ;
Laurencin-Dalicieux, Sara ;
Rue, Jacqueline ;
Farges, Jean-Christophe ;
Gennero, Isabelle ;
Conte-Auriol, Francoise ;
Briand-Mesange, Fabienne ;
Gadelorge, Melanie ;
Arzate, Higinio ;
Narayanan, A. Sampath ;
Brunel, Gerard ;
Salles, Jean-Pierre .
CELL AND TISSUE RESEARCH, 2007, 329 (02) :283-294
[10]   Raman and CARS microspectroscopy of cells and tissues [J].
Krafft, Christoph ;
Dietzek, Benjamin ;
Popp, Juergen .
ANALYST, 2009, 134 (06) :1046-1057
12