EspC, an autotransporter protein secreted by enteropathogenic Escherichia coli (EPEC), displays protease activity on human hemoglobin

被引：27

作者：

Drago-Serrano, Maria Elisa

Parra, Sandra Gavilanes

Manjarrez-Hernandez, H. Angel ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Salud Publ, Fac Med, Mexico City 04510, DF, Mexico

[2] UAM Xochimilco, Dept Sistemas Biol, Mexico City, DF, Mexico

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 265卷 / 01期

关键词：

EspC autotransporter protein; hemoglobin proteolysis; hemin-binding proteins; enteropathogenic Escherichia coli;

D O I：

10.1111/j.1574-6968.2006.00463.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Some enteropathogenic Escherichia coli (EPEC) strains, which are an important cause of diarrhea among infants, secrete a serine protease autotransporter protein called EspC. The pathogenic role of EspC upon EPEC infection is unknown. In this study, we demonstrated that purified EspC protein, obtained from supernatants of EPEC cultures, interacted with hemoglobin and degraded it. Moreover, we have shown that EspC is a hemin-binding protein. We hypothesized that hemoglobin proteolysis by EspC may contribute to the utilization of heme and hemoglobin iron for bacterial growth.

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页码：35 / 40

页数：6

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