A pharmacological approach in newly established retinal vein occlusion model

被引:43
作者
Fuma, Shinichiro [1 ]
Nishinaka, Anri [1 ]
Inoue, Yuki [1 ]
Tsuruma, Kazuhiro [1 ]
Shimazawa, Masamitsu [1 ]
Kondo, Mineo [2 ]
Hara, Hideaki [1 ]
机构
[1] Gifu Pharmaceut Univ, Dept Biofunct Evaluat, Mol Pharmacol, Gifu, Japan
[2] Mie Univ, Grad Sch Med, Dept Ophthalmol, Tsu, Mie, Japan
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词
ENDOTHELIAL GROWTH-FACTOR; MACULAR EDEMA; GENE-EXPRESSION; NATURAL-HISTORY; RAT MODEL; BEVACIZUMAB; ISCHEMIA; CELLS; NONPERFUSION; AQUAPORIN-4;
D O I
10.1038/srep43509
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mechanism underlying the effects of anti-vascular endothelial growth factor ( VEGF) antibody in retinal vein occlusion (RVO) treatment is poorly understood, partly due to the lack of RVO animal models that mimic clinical pathology. The aims of this study were to establish a suitable RVO model, clarify the pathogenic mechanisms, and evaluate the effects of anti-VEGF antibody in the model. Mouse retinal veins were occluded by laser photocoagulation after rose bengal injection. Reduction of the b/a wave amplitude ratio, retinal nonperfusion, cystoid edema, and hard exudates were observed after occlusion, and expression of RVO-related genes was altered. Administration of anti-VEGF antibody immediately, or 7 days, after occlusion resulted in reduction and increase of the nonperfused area, respectively. We conclude that the present model will be useful for clarification of the pathogenic mechanisms, and that the timing of anti-VEGF antibody administration is important for the successful amelioration of retinal nonperfusion.
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页数:14
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