A Method to Quantify Mouse Coat-Color Proportions

被引:6
作者
Ounpraseuth, Songthip
Rafferty, Tonya M.
McDonald-Phillips, Rachel E.
Gammill, Whitney M.
Siegel, Eric R.
Wheeler, Kristin L.
Nilsson, Erik A.
Cooney, Craig A.
机构
[1] Department of Biostatistics, The University of Arkansas for Medical Sciences, Little Rock, AR
[2] Department of Biochemistry and Molecular Biology, The University of Arkansas for Medical Sciences, Little Rock, AR
[3] Central Arkansas Veterans Healthcare Systems, Little Rock, AR
[4] Department of Pharmacology and Toxicology, The University of Arkansas for Medical Sciences, Little Rock, AR
[5] University of Arkansas, Fayetteville, AR
[6] Rhodes College, Memphis, TN
[7] Department of Computer and Information Science, Linköping University, Linköping
来源
PLOS ONE | 2009年 / 4卷 / 04期
关键词
AGOUTI GENE-EXPRESSION; GERM-LINE TRANSMISSION; EMBRYONIC STEM-CELLS; DNA METHYLATION; MUTATIONS; PHENOTYPE; AGREEMENT; GENOME; SITES; LOCUS;
D O I
10.1371/journal.pone.0005414
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Coat-color proportions and patterns in mice are used as assays for many processes such as transgene expression, chimerism, and epigenetics. In many studies, coat-color readouts are estimated from subjective scoring of individual mice. Here we show a method by which mouse coat color is quantified as the proportion of coat shown in one or more digital images. We use the yellow-agouti mouse model of epigenetic variegation to demonstrate this method. We apply this method to live mice using a conventional digital camera for data collection. We use a raster graphics editing program to convert agouti regions of the coat to a standard, uniform, brown color and the yellow regions of the coat to a standard, uniform, yellow color. We use a second program to quantify the proportions of these standard colors. This method provides quantification that relates directly to the visual appearance of the live animal. It also provides an objective analysis with a traceable record, and it should allow for precise comparisons of mouse coats and mouse cohorts within and between studies.
引用
收藏
页数:8
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