Backbone NMR resonance assignment of the catalytic subunit of cAMP-dependent protein kinase A in complex with AMP-PNP

被引：12
|
作者：
Masterson, Larry R. [1 ]
Shi, Lei [1 ]
Tonelli, Marco [2 ]
Mascioni, Alessandro [3 ]
Mueller, Michael M. [1 ]
Veglia, Gianluigi [1 ]
机构：
[1] Univ Minnesota, Dept Chem & Biochem, Minneapolis, MN 55455 USA
[2] Univ Wisconsin, Natl Magnet Resonance Facil Madison, Dept Biochem, Madison, WI 53706 USA
[3] Wyeth Pharmaceut, Andover, MA 01810 USA
来源：
BIOMOLECULAR NMR ASSIGNMENTS | 2009年 / 3卷 / 01期
关键词：
Protein kinase A; NMR; Protein backbone resonance assignments; Dual amino acid selective labeling; CCLS-HSQC; DYNAMICS;
D O I：
10.1007/s12104-009-9154-8
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The catalytic subunit of protein kinase A is involved with a number of signal transduction pathways and has been used as a benchmark to study the structural biology and biochemistry for the entire kinase family of enzymes. Here, we report the backbone assignment of the intact 41 kDa catalytic subunit bound to AMP-PNP.
引用
收藏
页码：115 / 117
页数：3
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