T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification

被引：393
作者：
Yamaguchi, TP
Takada, S
Yoshikawa, Y
Wu, NY
McMahon, AP [1 ]
机构：
[1] Harvard Univ, Biol Labs, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
[2] Kyoto Univ, Grad Sch Sci, Ctr Mol & Dev Biol, Sakyo Ku, Kyoto 6068502, Japan
[3] Japan Sci & Technol Corp, ERATO, Kondoh Differentiat Signaling Project, Sakyo Ku, Kyoto 6068305, Japan
[4] Kyoto Univ, Grad Sch Med, Dept Dermatol, Sakyo Ku, Kyoto 6068501, Japan
来源：
GENES & DEVELOPMENT | 1999年 / 13卷 / 24期
关键词：
gastrulation; mesoderm; neural; cell fate; Wnt; Brachyury;
D O I：
10.1101/gad.13.24.3185
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6, also lack paraxial mesoderm. Here we show that Brachyury is specifically down-regulated in Wnt3a mutants in cells fated to form paraxial mesoderm. Transgenic analysis of the T promoter identifies T (Brachyury) as a direct transcriptional target of the Wnt signaling pathway. Our results suggest that Wnt3a, signaling via Brachyury, modulates a balance between mesodermal and neural cell fates during gastrulation.
引用
收藏
页码：3185 / 3190
页数：6
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