Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans

被引：8

作者：

Feng, Qian ^{[1
]}

Hu, Zhuo-Ying ^{[2
]}

Liu, Xue-Qing ^{[1
]}

Zhang, Xue ^{[1
]}

Lan, Xi ^{[1
]}

Geng, Yan-Qing ^{[1
]}

Chen, Xue-Mei ^{[1
]}

He, Jun-Lin ^{[1
]}

Wang, Ying-Xiong ^{[1
]}

Ding, Yu-Bin ^{[1
]}

机构：

[1] Chongqing Med Univ, Sch Publ Hlth & Management, Reprod Biol Lab, Box 197,1 Yixueyuan Rd, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynaecol, Bldg 5 Room 507,1 Youyi Rd, Chongqing 400016, Peoples R China

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2017年 / 34卷 / 02期

基金：

中国国家自然科学基金;

关键词：

embryo implantation; decidualization; SLP-2; stromal cells; SLP-2; OVEREXPRESSION; POOR-PROGNOSIS; IMPLANTATION; CANCER; CARCINOMA; IDENTIFICATION; DEFECTS; GROWTH;

D O I：

10.1016/j.rbmo.2016.11.009

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The molecular mechanisms underlying endometrial stromal cell proliferation and differentiation (decidualization) are still not fully understood. This study revealed that increased Slp-2 expression is a significant factor modulating endometrial stromal cell proliferation and decidualization in both mice and humans. Our results showed a significant difference in the mRNA and protein levels between the implantation site and inter-implantation site on day 5 and day 6 of pregnancy in mice (all P < 0.05). Strong Slp-2 immunostaining was mainly localized within the decidual zone of mice through the post-implantation period. Mice with artificially induced deciduoma showed significantly higher expression of Slp-2 compared with uninduced controls (P < 0.005). Human stromal cells in the middle and late-secretory phases demonstrated significantly (all P < 0.05) upregulated SLP-2, compared with cells in the proliferative phase and early secretory phases. Further analyses of the SLP-2 gene knocked down revealed a significant (P < 0.005) repression of both the decidualization marker gene's expression (decidual/trophoblast prolactin-related protein in mice, insulin-like growth factor binding protein and prolactin in human) and the cell proliferation in in vitro-induced decidualized primary endometrial stromal cells in mice and humans. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

引用

页码：191 / 202

页数：12

共 29 条

[1] Post-implantation mouse conceptuses produce paracrine signals that regulate the uterine endometrium undergoing decidualization [J].