Nucleocytoplasmic Traffic of CPEB1 and Accumulation in Crm1 Nucleolar Bodies

被引:38
作者
Ernoult-Lange, Michele [1 ]
Wilczynska, Ania [1 ,2 ]
Harper, Maryannick [1 ]
Aigueperse, Christelle [1 ]
Dautry, Francois [1 ]
Kress, Michel [1 ]
Weil, Dominique [1 ]
机构
[1] Inst Andre Lwoff, CNRS, FRE2937, F-94801 Villejuif, France
[2] Warsaw Canc Ctr, Dept Mol Biol, PL-02781 Warsaw, Poland
关键词
NUCLEAR EXPORT SIGNALS; 60S RIBOSOMAL-SUBUNITS; BINDING PROTEIN; MESSENGER-RNA; PERINUCLEOLAR COMPARTMENT; LOCALIZATION; IDENTIFICATION; STRESS; TIAR; RIBONUCLEOPROTEIN;
D O I
10.1091/mbc.E08-09-0904
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The translational regulator CPEB1 plays a major role in the control of maternal mRNA in oocytes, as well as of subsynaptic mRNAs in neurons. Although mainly cytoplasmic, we found that CPEB1 protein is continuously shuttling between nucleus and cytoplasm. Its export is controlled by two redundant NES motifs dependent on the nuclear export receptor Crm1. In the nucleus, CPEB1 accumulates in a few foci most often associated with nucleoli. These foci are different from previously identified nuclear bodies. They contain Crm1 and were called Crm1 nucleolar bodies (CNoBs). CNoBs depend on RNA polymerase I activity, indicating a role in ribosome biogenesis. However, although they form in the nucleolus, they never migrate to the nuclear envelope, precluding a role as a mediator for ribosome export. They could rather constitute a platform providing factors for ribosome assembly or export. The behavior of CPEB1 in CNoBs raises the possibility that it is involved in ribosome biogenesis.
引用
收藏
页码:176 / 187
页数:12
相关论文
共 33 条
[1]   PHAX and CRM1 are required sequentially to transport U3 snoRNA to nucleoli [J].
Boulon, S ;
Verheggen, C ;
Jady, BE ;
Girard, C ;
Pescia, C ;
Paul, C ;
Ospina, JK ;
Kiss, T ;
Matera, AG ;
Bordonné, M ;
Bertrand, E .
MOLECULAR CELL, 2004, 16 (05) :777-787
[2]   ASSOCIATION OF POLYOMA T-ANTIGEN AND DNA WITH THE NUCLEAR MATRIX FROM LYTICALLY INFECTED 3T6-CELLS [J].
BUCKLERWHITE, AJ ;
HUMPHREY, GW ;
PIGIET, V .
CELL, 1980, 22 (01) :37-46
[3]   High frequency trans-splicing in a cell line producing spliced and polyadenylated RNA polymerase I transcripts from an rDNA-myc chimeric gene [J].
Chen, C ;
Fossar, N ;
Weil, D ;
Guillaud-Bataille, M ;
Danglot, G ;
Raynal, B ;
Dautry, F ;
Bernheim, A ;
Brison, O .
NUCLEIC ACIDS RESEARCH, 2005, 33 (07) :2332-2342
[4]   Identification and functional outcome of mRNAs associated with RNA-binding protein TIA-1 [J].
de Silanes, IL ;
Galbán, S ;
Martindale, JL ;
Yang, XL ;
Mazan-Mamczarz, K ;
Indig, FE ;
Falco, G ;
Zhan, M ;
Gorospe, M .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (21) :9520-9531
[5]   The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals [J].
Eberhart, DE ;
Malter, HE ;
Feng, Y ;
Warren, ST .
HUMAN MOLECULAR GENETICS, 1996, 5 (08) :1083-1091
[6]   Dual localization of the RNA binding protein CUGBP-1 to stress granule and perinucleolar compartment [J].
Fujimura, Ken ;
Kano, Fumi ;
Murata, Masayuki .
EXPERIMENTAL CELL RESEARCH, 2008, 314 (03) :543-553
[7]   Nuclear export of 60S ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p that associates with the large subunit protein Rpl10p [J].
Gadal, O ;
Strauss, D ;
Kessl, J ;
Trumpower, B ;
Tollervey, D ;
Hurt, E .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (10) :3405-3415
[8]   The dynamic organization of the perinucleolar compartment in the cell nucleus [J].
Huang, S ;
Deerinck, TJ ;
Ellisman, MH ;
Spector, DL .
JOURNAL OF CELL BIOLOGY, 1997, 137 (05) :965-974
[9]   IDENTIFICATION OF MOLECULAR CONTACTS BETWEEN THE U1-A SMALL NUCLEAR RIBONUCLEOPROTEIN AND U1 RNA [J].
JESSEN, TH ;
OUBRIDGE, C ;
TEO, CH ;
PRITCHARD, C ;
NAGAI, K .
EMBO JOURNAL, 1991, 10 (11) :3447-3456
[10]   CPEB1 regulates β-catenin mRNA translation and cell migration in astrocytes [J].
Jones, Kendrick J. ;
Korb, Erica ;
Kundel, Mitchell A. ;
Kochanek, Ashley R. ;
Kabraji, Sheheryar ;
McEvoy, Michael ;
Shin, Chan Y. ;
Wells, David G. .
GLIA, 2008, 56 (13) :1401-1413