Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin

被引:66
作者
Dou, YL [1 ]
Bowen, J [1 ]
Liu, YF [1 ]
Gorovsky, MA [1 ]
机构
[1] Univ Rochester, Dept Biol, Rochester, NY 14627 USA
关键词
H1; phosphorylation; ATP remodeling; FRAP; Tetrahymena;
D O I
10.1083/jcb.200202131
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In Tetrahymena cells, phosphorylation of linker histone HI regulates transcription of specific genes. Phosphorylation acts by creating a localized negative charge patch and phenocopies the loss of H1 from chromatin, suggesting that it affects transcription by regulating the dissociation of H1 from chromatin. To test this hypothesis, we used FRAP of GFP-tagged H1 to analyze the effects of mutations that either eliminate or mimic phosphorylation on the binding of H1 to chromatin both in vivo and in vitro. We demonstrate that phosphorylation can increase the rate of dissociation of H1 from chromatin, providing a mechanism by which it can affect HI function in vivo. We also demonstrate a previously undescribed ATP-dependent process that has a global effect on the dynamic binding of linker histone to chromatin.
引用
收藏
页码:1161 / 1170
页数:10
相关论文
共 75 条
[1]   What does 'chromatin remodeling' mean? [J].
Aalfs, JD ;
Kingston, RE .
TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (11) :548-555
[2]   Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H [J].
Aalfs, JD ;
Narlikar, GJ ;
Kingston, RE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (36) :34270-34278
[3]  
Ausubel F.M., 1988, CURRENT PROTOCOLS MO
[4]   MOBILITY MEASUREMENT BY ANALYSIS OF FLUORESCENCE PHOTOBLEACHING RECOVERY KINETICS [J].
AXELROD, D ;
KOPPEL, DE ;
SCHLESSINGER, J ;
ELSON, E ;
WEBB, WW .
BIOPHYSICAL JOURNAL, 1976, 16 (09) :1055-1069
[5]   Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span [J].
Barra, JL ;
Rhounim, L ;
Rossignol, JL ;
Faugeron, G .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (01) :61-69
[6]   Nucleosomes, linker DNA, and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin [J].
Bednar, J ;
Horowitz, RA ;
Grigoryev, SA ;
Carruthers, LM ;
Hansen, JC ;
Koster, AJ ;
Woodcock, CL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) :14173-14178
[7]   Low incidence of endometrial hyperplasia with acceptable bleeding patterns in women taking sequential hormone replacement therapy with dydrogesterone [J].
Bergeron, C ;
Fox, H .
GYNECOLOGICAL ENDOCRINOLOGY, 2000, 14 (04) :275-281
[8]   Activation of RNA polymerase II transcription [J].
Berk, AJ .
CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (03) :330-335
[9]   Effect of ATP depletion on the palmitoylation of myelin proteolipid protein in young and adult rats [J].
Bizzozero, OA ;
Sanchez, P ;
Tetzloff, SU .
JOURNAL OF NEUROCHEMISTRY, 1999, 72 (06) :2610-2616
[10]   ELECTROSTATIC MECHANISM OF NUCLEOSOME SPACING [J].
BLANK, TA ;
BECKER, PB .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 252 (03) :305-313