Immobilization of pectin depolymerising polygalacturonase using different polymers

被引：53

作者：

Rehman, Haneef Ur ^{[1
]}

Aman, Afsheen ^{[2
]}

Nawaz, Muhammad Asif ^{[3
]}

Karim, Asad ^{[2
]}

Ghani, Maria ^{[2
]}

Baloch, Abdul Hameed ^{[4
]}

Ul Qader, Shah Ali ^{[2
]}

机构：

[1] Univ Turbat, Dept Chem, Kech, Balochistan, Pakistan

[2] Univ Karachi, KIBGE, Karachi 75270, Pakistan

[3] Shaheed Benazir Bhutto Univ, Dept Biotechnol, Dir Upper, Kpk, Pakistan

[4] LUAWMS, Fac Agr, Uthal 90150, Balochistan, Pakistan

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 82卷

关键词：

Polygalacturonase; Immobilization; Entrapment; Calcium alginate beads; Agar-agar matrix; Polyacrylamide gel; KIBGE IB-21; APPLE JUICE; SUPPORT; ENZYME; STABILIZATION; ALGINATE; CHITOSAN; CALCIUM; AGAR;

D O I：

10.1016/j.ijbiomac.2015.10.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polygalacturonase catalyses the hydrolysis of pectin substances and widely has been used in food and textile industries. In current study, different polymers such as calcium alginate beads, polyacrylamide gel and agar-agar matrix were screened for the immobilization of polygalacturonase through entrapment technique. Polyacrylamide gel was found to be most promising one and gave maximum (89%) immobilization yield as compared to agar-agar (80%) and calcium alginate beads (46%). The polymers increased the reaction time of polygalacturonase and polymers entrapped polygalacturonases showed maximum pectinolytic activity after 10 min of reaction as compared to free polygalacturonase which performed maximum activity after 5.0 min of reaction time. The temperature of polygalacturonase for maximum enzymatic activity was increased from 45 degrees C to 50 degrees C and 55 degrees C when it was immobilized within agar-agar and calcium alginate beads, respectively. The optimum pH (pH 10) of polygalacturonase was remained same when it was immobilized within polyacrylamide gel and calcium alginate beads, but changed from pH 10 to pH 9.0 after entrapment within agar-agar. Thermal stability of polygalacturonase was improved after immobilization and immobilized polygalacturonases showed higher tolerance against different temperatures as compared to free enzyme. Polymers entrapped polygalacturonases showed good reusability and retained more than 80% of their initial activity during 2nd cycles. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：127 / 133

页数：7

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