Imaging Mass Cytometry

被引：196

作者：

Chang, Qing ^{[1
]}

Ornatsky, Olga I. ^{[1
]}

Siddiqui, Iram ^{[2
]}

Loboda, Alexander ^{[1
]}

Baranov, Vladimir I. ^{[1
]}

Hedley, David W. ^{[3
]}

机构：

[1] Fluidigm Canada Inc, Markham, ON L3R 4G5, Canada

[2] Hosp Sick Children, Dept Pathol, Toronto, ON M5G 1X8, Canada

[3] Princess Margaret Canc Ctr, Div Med Oncol & Hematol, 610 Univ Ave, Toronto, ON M5G 2M9, Canada

来源：

CYTOMETRY PART A | 2017年 / 91A卷 / 02期

关键词：

mass cytometry; image analysis; systems biology; hypoxia; cell signaling; SPECTROMETRY; RESOLUTION; HYPOXIA; TISSUE; CELLS; TUMOR; IDENTIFICATION; HETEROGENEITY;

D O I：

10.1002/cyto.a.23053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Imaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy, with the high content of mass cytometry enabled through the use of isotopically labeled probes and ICP-MS detection. Importantly, IMC can be performed on paraffin-embedded tissue sections, so can be applied to the retrospective analysis of patient cohorts whose outcome is known, and eventually to personalized medicine. Since the original description in 2014, IMC has evolved rapidly into a commercial instrument of unprecedented power for the analysis of histological sections. In this Review, we discuss the underlying principles of this new technology, and outline emerging applications of IMC in the analysis of normal and pathological tissues. (c) 2017 International Society for Advancement of Cytometry

引用

收藏

页码：160 / 169

页数：10

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