Dysregulated miR-671-5p/CDR1-AS/CDR1/VSNL1 axis is involved in glioblastoma multiforme

被引：107

作者：

Barbagallo, Davide ^{[1
]}

Condorelli, Angelo ^{[1
]}

Ragusa, Marco ^{[1
]}

Salito, Loredana ^{[1
]}

Sammito, Mariangela ^{[1
]}

Banelli, Barbara ^{[2
]}

Caltabiano, Rosario ^{[3
]}

Barbagallo, Giuseppe ^{[3
]}

Zappala, Agata ^{[4
]}

Battaglia, Rosalia ^{[1
]}

Cirnigliaro, Matilde ^{[1
]}

Lanzafame, Salvatore ^{[3
]}

Vasquez, Enrico ^{[3
]}

Parenti, Rosalba ^{[4
]}

Cicirata, Federico ^{[4
]}

Di Pietro, Cinzia ^{[1
]}

Romani, Massimo ^{[2
]}

Purrello, Michele ^{[1
]}

机构：

[1] Univ Catania, Unita BioMed Mol Genom & Sistemi Complessi, Dipartimento Sci Biomed & Biotecnol, Sez Biol & Genet G Sichel, Catania, Italy

[2] IRCCS AOU San Martino IST, UOS Epigenet Tumori, Genoa, Italy

[3] Univ Catania, Dipartimento Sci Med Chirurg & Tecnol Avanzate GF, Catania, Italy

[4] Univ Catania, Sez Fisiol, Dipartimento Sci Biomed & Biotecnol, Catania, Italy

来源：

ONCOTARGET | 2016年 / 7卷 / 04期

关键词：

glioblastoma multiforme (GBM); non coding RNAs (ncRNAs); microRNAs (miRNAs); circular RNAs (circRNAs); cell networks; GLOBAL-NETWORK STRUCTURE; MICRORNA TRANSCRIPTOME; MESSENGER-RNA; CIRCULAR RNAS; EXPRESSION; GLIOMA; CELLS; TEMOZOLOMIDE; INTEGRATION; PREDICTION;

D O I：

10.18632/oncotarget.6621

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR-671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.

引用

页码：4746 / 4759

页数：14

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