Rad6-dependent ubiquitination of histone H2B in yeast

被引：523

作者：

Robzyk, K ^{[1
]}

Recht, L ^{[1
]}

Osley, MA ^{[1
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA

来源：

SCIENCE | 2000年 / 287卷 / 5452期

关键词：

D O I：

10.1126/science.287.5452.501

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although ubiquitinated histones are present in substantial Levels in vertebrate cells, the roles they play in specific biological processes and the cellular factors that regulate this modification are not well characterized. Ubiquitinated H2B (uH2B) has been identified in the yeast Saccharomyces cerevisiae, and mutation of the conserved ubiquitination site is shown to confer defects in mitotic cell growth and meiosis. uH2B was not detected in rad6 mutants, which are defective for the ubiquitin-conjugating enzyme Ubc2, thus identifying Rad6 as the major cellular activity that ubiquitinates H2B in yeast.

引用

页码：501 / 504

页数：4

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