Potential applications of three-dimensional structure of silk fibroin/poly(ester-urethane) urea nanofibrous scaffold in heart valve tissue engineering

被引：40

作者：

Du, Juan ^{[1
]}

Zhu, Tonghe ^{[2
]}

Yu, Haiyan ^{[1
]}

Zhu, Jingjing ^{[2
]}

Sun, Changbing ^{[1
]}

Wang, Jincheng ^{[1
]}

Chen, Sihao ^{[1
]}

Wang, Jihu ^{[1
]}

Guo, Xuran ^{[2
]}

机构：

[1] Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China

[2] Donghua Univ, Coll Chem Chem Engn & Biotechnol, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

来源：

APPLIED SURFACE SCIENCE | 2018年 / 447卷

关键词：

Silk fibroin; Poly(ester-urethane) urea elastomer; Nanofiber; Electrospinning; Heart valve tissue engineering; BIOMEDICAL APPLICATIONS; MECHANICAL-PROPERTIES; SPIDER SILK; FIBROIN; FABRICATION; FIBERS; POLYURETHANE; HYDROGELS; MATRIX; ENDOTHELIALIZATION;

D O I：

10.1016/j.apsusc.2018.03.077

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Tissue engineering heart valves (TEHV) are thought to have many advantages in low immunogenicity, good histocompatibility, excellent mechanical properties. In this paper, we reported the fabrication and characterization of a novel composite nanofibrous scaffold consisting of silk fibroin (SF) and poly (ester-urethane) urea (LDI-PEUU) by using electrospinning. Chemical and physical properties of scaffolds were evaluated using scanning electron microscopy, attenuated total reflectance Fourier transform infrared, X-ray diffraction, contact angle measurement, thermogravimetric analysis, biodegradation test and tensile strength analysis. We determined that the composite scaffolds supported the growth of human umbilical vein endothelial cell (HUVEC). The results of cell proliferation and cell morphology indicate that SF/LDI-PEUU nanofibers promoted cell viability, which supporting the application in tissue engineering. All results clarified that SF/LDI-PEUU (40:60) nanofibrous scaffolds meet the required specifications for tissue engineering and could be used as a promising construct for heart valve tissue engineering. (C) 2018 Published by Elsevier B.V.

引用

页码：269 / 278

页数：10

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