Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

被引：83

作者：

Hino, M ^{[1
]}

Kurogi, K ^{[1
]}

Okubo, MA ^{[1
]}

Murata-Hori, M ^{[1
]}

Hosoya, H ^{[1
]}

机构：

[1] Hiroshima Univ, Fac Sci, Dept Biol Sci, Higashihiroshima 7398526, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 271卷 / 01期

关键词：

small heat shock protein; HSP27; microtubules; tubulin; mitotic spindle; monoclonal antibody;

D O I：

10.1006/bbrc.2000.2553

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.

引用

页码：164 / 169

页数：6

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