Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

被引：83
|
作者：
Hino, M [1 ]
Kurogi, K [1 ]
Okubo, MA [1 ]
Murata-Hori, M [1 ]
Hosoya, H [1 ]
机构：
[1] Hiroshima Univ, Fac Sci, Dept Biol Sci, Higashihiroshima 7398526, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 271卷 / 01期
关键词：
small heat shock protein; HSP27; microtubules; tubulin; mitotic spindle; monoclonal antibody;
D O I：
10.1006/bbrc.2000.2553
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.
引用
收藏
页码：164 / 169
页数：6
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