The influence of serum-supplemented culture media in a transwell migration assay

被引：38

作者：

Zaki, Siti Sarah Omar ^{[1
]}

Kanesan, Livashini ^{[1
]}

Leong, Ming Yeh Danielle ^{[1
,2
]}

Vidyadaran, Sharmili ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Pathol, Neuroinflammat Grp,Immunol Lab, Serdang 43400, Malaysia

[2] Int Med Univ, Sch Hlth Sci, Div Appl Biomed Sci & Biotechnol, 126,Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia

来源：

CELL BIOLOGY INTERNATIONAL | 2019年 / 43卷 / 10期

关键词：

BV2; microglia; chemoattractant; serum; transwell migration assay; MICROGLIAL CELLS; CANCER; ATP;

D O I：

10.1002/cbin.11122

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.

引用

页码：1201 / 1204

页数：4