The influence of serum-supplemented culture media in a transwell migration assay

被引：37
|
作者：
Zaki, Siti Sarah Omar [1 ]
Kanesan, Livashini [1 ]
Leong, Ming Yeh Danielle [1 ,2 ]
Vidyadaran, Sharmili [1 ]
机构：
[1] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Pathol, Neuroinflammat Grp,Immunol Lab, Serdang 43400, Malaysia
[2] Int Med Univ, Sch Hlth Sci, Div Appl Biomed Sci & Biotechnol, 126,Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia
来源：
CELL BIOLOGY INTERNATIONAL | 2019年 / 43卷 / 10期
关键词：
BV2; microglia; chemoattractant; serum; transwell migration assay; MICROGLIAL CELLS; CANCER; ATP;
D O I：
10.1002/cbin.11122
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
引用
收藏
页码：1201 / 1204
页数：4
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