HSP70 expression in Biomphalaria glabrata snails exposed to cadmium

被引：14

作者：

Cantinha, Rebeca da Silva ^{[1
,2
]}

Borrely, Sueli Ivone ^{[2
]}

Oguiura, Nancy ^{[1
]}

de Braganca Pereira, Carlos Alberto ^{[3
]}

Rigolon, Marcela M. ^{[1
]}

Nakano, Eliana ^{[1
]}

机构：

[1] Inst Butantan, Parasitol Lab, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil

[2] Inst Pesquisas Energet & Nucl, Ave Prof Lineu Prestes 2242,Cidade Univ, BR-05508000 Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Inst Matemat & Estadst, Dept Estat, Rua Matao,1010 Cidade Univ, BR-05008090 Sao Paulo, SP, Brazil

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2017年 / 140卷

关键词：

Protein induction; Biomarker; Bioindicator; Mollusk; Acquired resistance; HEAT-SHOCK-PROTEIN; CRASSOSTREA-VIRGINICA; STRESS-PROTEINS; BIOMARKER; WATER; HEAT-SHOCK-PROTEIN-70; TOLERANCE; RESPONSES; METALS; MUSSEL;

D O I：

10.1016/j.ecoenv.2017.02.026

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96 h to CdCl2 at concentrations ranging from 0.09 to 0.7 mg L-1 (LC50/96 (h) = 0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies.

引用

页码：18 / 23

页数：6

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