HSP70 expression in Biomphalaria glabrata snails exposed to cadmium

被引：14

作者：

Cantinha, Rebeca da Silva ^{[1
,2
]}

Borrely, Sueli Ivone ^{[2
]}

Oguiura, Nancy ^{[1
]}

de Braganca Pereira, Carlos Alberto ^{[3
]}

Rigolon, Marcela M. ^{[1
]}

Nakano, Eliana ^{[1
]}

机构：

[1] Inst Butantan, Parasitol Lab, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP, Brazil

[2] Inst Pesquisas Energet & Nucl, Ave Prof Lineu Prestes 2242,Cidade Univ, BR-05508000 Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Inst Matemat & Estadst, Dept Estat, Rua Matao,1010 Cidade Univ, BR-05008090 Sao Paulo, SP, Brazil

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2017年 / 140卷

关键词：

Protein induction; Biomarker; Bioindicator; Mollusk; Acquired resistance; HEAT-SHOCK-PROTEIN; CRASSOSTREA-VIRGINICA; STRESS-PROTEINS; BIOMARKER; WATER; HEAT-SHOCK-PROTEIN-70; TOLERANCE; RESPONSES; METALS; MUSSEL;

D O I：

10.1016/j.ecoenv.2017.02.026

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96 h to CdCl2 at concentrations ranging from 0.09 to 0.7 mg L-1 (LC50/96 (h) = 0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies.

引用

页码：18 / 23

页数：6

共 50 条

[1] Abd Allah AT, 2003, J MOLLUS STUD, V69, P35, DOI 10.1093/mollus/69.1.35
[2] Potential use of glycogen level as biomarker of chemical stress in Biomphalaria glabrata
Ansaldo, Martin
Nahabedian, Daniel E.
Holmes-Brown, Eduardo
Agote, Marcos
Ansay, Cristina V.
Verrengia Guerrero, Noemi R.
Wider, Eva A.
[J]. TOXICOLOGY, 2006, 224 (1-2) : 119 - 127
[3] Barlow R.E., 1998, Engineering Reliability
[4] Modulation of the response to stress factors of Xerolycosa nemoralis (Lycosidae) spiders living in contaminated environments
Bednarek, Agata
Sawadro, Marta
Babczynska, Agnieszka
[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2016, 131 : 1 - 6
[5] Applications and pitfalls of stress-proteins in biomonitoring
Bierkens, JGEA
[J]. TOXICOLOGY, 2000, 153 (1-3) : 61 - 72
[6] Cantinha Borrely S. I., 2013, GREENER J CELL ANIM, V1, P1
[7] Biochemical biomarker responses of green-lipped mussel, Perna canaliculus, to acute and subchronic waterborne cadmium toxicity
Chandurvelan, Rathishri
Marsden, Islay D.
Gaw, Sally
Glover, Chris N.
[J]. AQUATIC TOXICOLOGY, 2013, 140 : 303 - 313
[8] Dominant lethal effects of 2,4-D in Biomphalaria glabrata
Estevam, Ethiene Castellucci
Nakano, Eliana
Kawano, Toshie
de Braganca Pereira, Carlos Alberto
Amancio, Francisco Fernandes
de Albuquerque Melo, Ana Maria Mendonca
[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2006, 611 (1-2) : 83 - 88
[9] Friberg L., 1992, ENV HLTH CRIT, V134
[10] Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different HSP70s
Geraci, F
Pinsino, A
Turturici, G
Savona, R
Giudice, G
Sconzo, G
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 322 (03) : 873 - 877

← 1 2 3 4 5 →