Requirement of Poly(rC) binding protein 2 for translation of poliovirus RNA

被引：267

作者：

Blyn, LB

Towner, JS

Semler, BL

Ehrenfeld, E

机构：

[1] UNIV CALIF IRVINE,DEPT MICROBIOL & MOL GENET,IRVINE,CA 92697

[2] UNIV CALIF IRVINE,DEPT MOL BIOL & BIOCHEM,IRVINE,CA 92697

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 08期

关键词：

D O I：

10.1128/JVI.71.8.6243-6246.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Poly(rC) binding protein 2 (PCBP2) is one of several cellular proteins that interact specifically with a major stem-loop domain in the poliovirus internal ribosome entry site. HeLa cell extracts subjected to stem-loop IV RNA affinity chromatography were depleted of all detectable PCBP2. Such extracts were unable to efficiently translate poliovirus RNA, although extracts recovered from control columns of matrix unlinked to RNA retained full translation activity. Both translation and production of infectious progeny virus were restored in the PCBP2-depleted extracts by addition of recombinant PCBP2, but not by PCBP1, which is a closely related member of the protein family. The data show that PCBP2 is an essential factor, which is required for efficient translation of poliovirus RNA in HeLa cells.

引用

收藏

页码：6243 / 6246

页数：4

相关论文

共 25 条

[1] TISSUE-SPECIFIC EXPRESSION AND CDNA STRUCTURE OF A HUMAN TRANSCRIPT ENCODING A NUCLEIC-ACID BINDING [OLIGO(DC)] PROTEIN RELATED TO THE PRE-MESSENGER-RNA BINDING-PROTEIN-K [J].

AASHEIM, HC ;

LOUKIANOVA, T ;

DEGGERDAL, A ;

SMELAND, EB .

NUCLEIC ACIDS RESEARCH, 1994, 22 (06) :959-964

[2] COMPLETE REPLICATION OF POLIOVIRUS IN-VITRO - PREINITIATION RNA REPLICATION COMPLEXES REQUIRE SOLUBLE CELLULAR FACTORS FOR THE SYNTHESIS OF VPG-LINKED RNA [J].

BARTON, DJ ;

BLACK, EP ;

FLANEGAN, JB .

JOURNAL OF VIROLOGY, 1995, 69 (09) :5516-5527

[3] HOST-CELL PROTEINS BINDING TO DOMAIN-IV OF THE 5' NONCODING REGION OF POLIOVIRUS RNA [J].

BLYN, LB ;

CHEN, RY ;

SEMLER, BL ;

EHRENFELD, E .

JOURNAL OF VIROLOGY, 1995, 69 (07) :4381-4389

[4] Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: Identification by automated liquid chromatography tandem mass spectrometry [J].

Blyn, LB ;

Swiderek, KM ;

Richards, O ;

Stahl, DC ;

Semler, BL ;

Ehrenfeld, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :11115-11120

[5] CONSERVATION OF RNA-PROTEIN INTERACTIONS AMONG PICORNAVIRUSES [J].

DILDINE, SL ;

SEMLER, BL .

JOURNAL OF VIROLOGY, 1992, 66 (07) :4364-4376

[6]

Ehrenfeld E, 1995, CURR TOP MICROBIOL, V203, P65

[7]

Ehrenfeld E., 1996, TRANSLATIONAL CONTRO, P549

[8] MINIMUM INTERNAL RIBOSOME ENTRY SITE REQUIRED FOR POLIOVIRUS INFECTIVITY [J].

HALLER, AA ;

NGUYEN, JHC ;

SEMLER, BL .

JOURNAL OF VIROLOGY, 1993, 67 (12) :7461-7471

[9] THE CELLULAR POLYPEPTIDE P57 (PYRIMIDINE TRACT-BINDING PROTEIN) BINDS TO MULTIPLE SITES IN THE POLIOVIRUS 5' NONTRANSLATED REGION [J].

HELLEN, CUT ;

PESTOVA, TV ;

LITTERST, M ;

WIMMER, E .

JOURNAL OF VIROLOGY, 1994, 68 (02) :941-950

[10] A CYTOPLASMIC 57-KDA PROTEIN THAT IS REQUIRED FOR TRANSLATION OF PICORNAVIRUS RNA BY INTERNAL RIBOSOMAL ENTRY IS IDENTICAL TO THE NUCLEAR PYRIMIDINE TRACT-BINDING PROTEIN [J].

HELLEN, CUT ;

WITHERELL, GW ;

SCHMID, M ;

SHIN, SH ;

PESTOVA, TV ;

GIL, A ;

WIMMER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (16) :7642-7646