Seasonal Stability and Dynamics of DNA Methylation in Plants in a Natural Environment

被引：25

作者：

Ito, Tasuku ^{[1
]}

Nishio, Haruki ^{[1
]}

Tarutani, Yoshiaki ^{[2
]}

Emura, Naoko ^{[1
,3
]}

Honjo, Mie N. ^{[1
]}

Toyoda, Atsushi ^{[4
,5
]}

Fujiyama, Asao ^{[5
]}

Kakutani, Tetsuji ^{[2
,6
]}

Kudoh, Hiroshi ^{[1
]}

机构：

[1] Kyoto Univ, Ctr Ecol Res, Otsu, Shiga 5202113, Japan

[2] Natl Inst Genet, Dept Chromosome Sci, Mishima, Shizuoka 4118540, Japan

[3] Kagoshima Univ, Fac Agr, Dept Environm Sci & Technol, Korimoto 1-21-24, Kagoshima 8900065, Japan

[4] Natl Inst Genet, Dept Genom & Evolutionary Biol, Mishima, Shizuoka 4118540, Japan

[5] Natl Inst Genet, Adv Genom Ctr, Mishima, Shizuoka 4118540, Japan

[6] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

GENES | 2019年 / 10卷 / 07期

关键词：

Arabidopsis halleri; DNA methylation; natural environment; seasonal changes; seasonally methylated cytosines; EPIGENETIC VARIATION; CG METHYLATION; TRANSPOSON; MUTATION;

D O I：

10.3390/genes10070544

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA methylation has been considered a stable epigenetic mark but may respond to fluctuating environments. However, it is unclear how they behave in natural environments. Here, we analyzed seasonal patterns of genome-wide DNA methylation in a single clone from a natural population of the perennial Arabidopsis halleri. The genome-wide pattern of DNA methylation was primarily stable, and most of the repetitive regions were methylated across the year. Although the proportion was small, we detected seasonally methylated cytosines (SeMCs) in the genome. SeMCs in the CHH context were detected predominantly at repetitive sequences in intergenic regions. In contrast, gene-body CG methylation (gbM) itself was generally stable across seasons, but the levels of gbM were positively associated with seasonal stability of RNA expression of the genes. These results suggest the existence of two distinct aspects of DNA methylation in natural environments: sources of epigenetic variation and epigenetic marks for stable gene expression.

引用

页数：13

共 61 条

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