MARKER ASSISTED IDENTIFICATION OF TOSPOVIRUS RESISTANT TOMATO GENOTYPES IN SEGREGATING PROGENIES

被引：3

作者：

do Nascimento, Ildon Rodrigues ^{[1
]}

Maluf, Wilson Roberto ^{[2
]}

Figueira, Antonia Reis ^{[3
]}

Menezes, Cicero Beserra ^{[4
]}

Vilela de Resende, Juliano Tadeu ^{[1
]}

Faria, Marcos Ventura ^{[1
]}

Nogueira, Douglas Willian ^{[2
]}

机构：

[1] UFT, BR-77402970 Gurupi, TO, Brazil

[2] Univ Fed Lavras, Depto Fitotecnia, BR-37200000 Lavras, MG, Brazil

[3] Univ Estadual Centro Oeste, Depto Agron, BR-85040080 Guarapuava, PR, Brazil

[4] Univ Fed Lavras, Depto Fitopatol, BR-37200000 Lavras, MG, Brazil

来源：

SCIENTIA AGRICOLA | 2009年 / 66卷 / 03期

关键词：

Lycopersicon esculentum; Sw-5; virus resistance; molecular marker; WILT-VIRUS-RESISTANCE;

D O I：

10.1590/S0103-90162009000300003

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5(+)) from susceptible (Sw-5(+)/Sw-5(+)) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5(+)), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.

引用

页码：298 / 303

页数：6

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