Metabolic engineering in the biotechnological production of organic acids in the tricarboxylic acid cycle of microorganisms: Advances and prospects

被引：184

作者：

Yin, Xian ^{[1
,2
,3
]}

Li, Jianghua ^{[1
,3
]}

Shin, Hyun-dong ^{[4
]}

Du, Guocheng ^{[1
,3
]}

Liu, Long ^{[1
,2
,3
]}

Chen, Jian ^{[1
,5
]}

机构：

[1] Jiangnan Univ, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi 214122, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[3] Synerget Innovat Ctr Food Safety & Nutr, Wuxi 214122, Peoples R China

[4] GA Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

[5] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China

来源：

BIOTECHNOLOGY ADVANCES | 2015年 / 33卷 / 06期

关键词：

Metabolic engineering; Tricarboxylic acid (TCA) cycle; Citric acid; alpha-Ketoglutaric acid; Succinic acid; Fumaric acid; L-Malic acid; Oxaloacetate; C-4-dicarboxylic acid; ALPHA-KETOGLUTARIC ACID; YARROWIA-LIPOLYTICA WSH-Z06; L-MALIC ACID; ESCHERICHIA-COLI-C; FUMARIC-ACID; ASPERGILLUS-NIGER; CITRIC-ACID; SUCCINATE PRODUCTION; PYRUVATE-CARBOXYLASE; CORYNEBACTERIUM-GLUTAMICUM;

D O I：

10.1016/j.biotechadv.2015.04.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Organic acids, which are chemically synthesized, are also natural intermediates in the metabolic pathways of microorganisms, among which the tricarboxylic acid (TCA) cycle is the most crucial route existing in almost all living organisms. Organic acids in the TCA cycle include citric acid, a-ketoglutaric acid, succinic acid, fumaric acid, L-malic acid, and oxaloacetate, which are building-block chemicals with wide applications and huge markets. In this review, we summarize the synthesis pathways of these organic acids and review recent advances in metabolic engineering strategies that enhance organic acid production. We also propose further improvements for the production of organic acids with systems and synthetic biology-guided metabolic engineering strategies. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：830 / 841

页数：12

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