Crystallization and preliminary X-ray crystallographic analysis of the DNA-binding domain of BldD from Streptomyces coelicolor A3(2)

被引：2

作者：

Kim, IK

Kim, MK

Lee, CJ

Yim, HS

Cha, SS ^{[1
]}

Kang, SO

机构：

[1] Seoul Natl Univ, Sch Biol Sci, Biophys Lab, Seoul 151742, South Korea

[2] Seoul Natl Univ, Inst Microbiol, Seoul 151742, South Korea

[3] Pohang Accelerator Lab, Beamline Div, Pohang 790784, Kyungbuk, South Korea

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904006961

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The N-terminal DNA-binding domain of BldD from Streptomyces coelicolor A3(2) was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.8 Angstrom data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 77.2, b = 31.8, c = 33.6 Angstrom, beta = 105.1degrees. Analysis of the packing density shows that the asymmetric unit probably contains one molecule, with a solvent content of 43.6%.

引用

页码：1115 / 1117

页数：3

共 19 条

[1] σBldN, an extracytoplasmic function RNA polymerase sigma factor required for aerial mycelium formation in Streptomyces coelicolor A3(2) [J].