Cloning of an Arabidopsis cDNA encoding a chloroplast localizing sulphate transporter isoform

被引：34
|
作者：
Takahashi, H [1 ]
Asanuma, W [1 ]
Saito, K [1 ]
机构：
[1] Chiba Univ, Fac Pharmaceut Sci, Res Ctr Med Resources, Lab Mol Biol & Biotechnol,Inage Ku, Chiba 2638522, Japan
来源：
JOURNAL OF EXPERIMENTAL BOTANY | 1999年 / 50卷 / 340期
关键词：
Arabidopsis thaliana; chloroplast; green fluorescent; protein; sulphate transporter;
D O I：
10.1093/jexbot/50.340.1713
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A new member of the sulphate transporter gene in Arabidopsis encoding a chloroplast-localizing isoform, AST82 (accession no. AB008782) has been identified, AST82 had a sequence similarity with a cyanobacterial genome sequence, slr1776, which encodes a putative eukaryotic proton/sulphate co-transporter. Fusion proteins of AST82 and green fluorescent protein (GFP) transiently expressed in Arabidopsis leaves localized in chloroplasts, indicating that AST82 encodes a chloroplastic isoform.
引用
收藏
页码：1713 / 1714
页数：2
相关论文
共 50 条
  • [1] Cloning and characterisation of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase
    Roberts, MA
    Wray, JL
    PLANT MOLECULAR BIOLOGY, 1996, 30 (05) : 1041 - 1049
  • [2] Cloning and in vitro expression of the cDNA encoding a putative nucleoside transporter from Arabidopsis thaliana
    Li, J
    Wang, DW
    PLANT SCIENCE, 2000, 157 (01) : 23 - 32
  • [3] Molecular cloning and characterization of a cDNA encoding proline transporter in rice
    Igarashi, Y
    Yoshiba, Y
    Takeshita, T
    Nomura, S
    Otomo, J
    Yamaguchi-Shinozaki, K
    Shinozaki, K
    PLANT AND CELL PHYSIOLOGY, 2000, 41 (06) : 750 - 756
  • [4] Cloning and characterization of a Drosophila melanogaster cDNA encoding a glutamate transporter
    Kawano, T
    Takuwa, K
    Kuniyoshi, H
    Juni, N
    Nakajima, T
    Yamamoto, D
    Kimura, Y
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (11) : 2042 - 2044
  • [5] Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana
    Tamura, K
    Nishiura, H
    Mori, J
    Imai, H
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 : 745 - 747
  • [6] Functional cloning of an Arabidopsis thaliana cDNA encoding cycloeucalenol cycloisomerase
    Lovato, MA
    Hart, EA
    Segura, MJR
    Giner, JL
    Matsuda, SPT
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (18) : 13394 - 13397
  • [7] Isolation and characterization of a cDNA encoding a sulfate transporter from Arabidopsis thaliana
    Takahashi, H
    Sasakura, N
    Noji, M
    Saito, K
    FEBS LETTERS, 1996, 392 (02) : 95 - 99
  • [8] Molecular cloning and characterization of a tobacco leaf cDNA encoding a phosphate transporter
    Baek, SH
    Chung, IM
    Yun, SJ
    MOLECULES AND CELLS, 2001, 11 (01) : 1 - 6
  • [9] Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana
    Katagiri, T
    Mizoguchi, T
    Shinozaki, K
    PLANT MOLECULAR BIOLOGY, 1996, 30 (03) : 647 - 653
  • [10] Cloning and expression of cDNA encoding heart-type isoform of AMP deaminase
    Wang, XD
    Morisaki, H
    Sermsuvitayawong, K
    Mineo, I
    Toyama, K
    Ogasawara, N
    Mukai, T
    Morisaki, T
    GENE, 1997, 188 (02) : 285 - 290
12345