Quantitative Matrix-Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance (MALDI-FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

被引：47

作者：

Stoop, Marcel P. ^{[1
]}

Dekker, Lennard J. ^{[1
]}

Titulaer, Mark K. ^{[1
]}

Lamers, Robert-Jan A. N. ^{[1
]}

Burgers, Peter C. ^{[1
]}

Smitt, Peter A. E. Sillevis ^{[1
]}

van Gool, Alain J. ^{[2
]}

Luider, Theo M. ^{[1
]}

Hintzen, Rogler Q. ^{[1
]}

机构：

[1] Erasmus Univ, Med Ctr, Dept Neurol, Labs Neurooncol Clin & Canc Prote, NL-3000 DR Rotterdam, Netherlands

[2] Schering Plough Corp, Target Discovery Unit, NL-5349 AM Oss, Netherlands

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 03期

关键词：

Cerebrospinal fluid; differentially abundant peptides; multiple sclerosis; proteomics; innate immunity; clinically isolated syndrome; CEREBROSPINAL-FLUID PROTEOME; C-REACTIVE PROTEIN; FREE KAPPA-LIGHT; MASS-SPECTROMETRY; NEUROLOGICAL DISEASES; CHROMOGRANIN-A; SPINAL-FLUID; CYSTATIN-C; FTICR-MS; BIOMARKERS;

D O I：

10.1021/pr8010155

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).

引用

页码：1404 / 1414

页数：11

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