Luminescent Terbium Protein Labels for Time-Resolved Microscopy and Screening

被引：65

作者：

Rajapakse, Harsha E. ^{[1
]}

Reddy, D. Rajasekhar ^{[1
]}

Mohandessi, Shabnam ^{[1
]}

Butlin, Nathaniel G. ^{[2
]}

Miller, Lawrence W. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Chicago, IL 60607 USA

[2] Lumiphore Inc, Richmond, CA 94804 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 27期

基金：

美国国家卫生研究院;

关键词：

FRET; lanthanides; protein labeling; time-resolved microscopy; trimethoprim; RESONANCE ENERGY-TRANSFER; LIVING CELLS; IN-VITRO; EUROPIUM; PROBES; COMPLEXES; FLUORESCENCE; CHELATE; SYSTEM; AGENTS;

D O I：

10.1002/anie.200900858

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Brilliance of terbium: Heterodimeric conjugates of trimethoprim covalently linked to sensitized terbium chelates bind to Escherichia coli dihydrofolate reductase fusion proteins with nanomolar affinity (see picture). Terbium luminescence enables sensitive and time-resolved detection of labeled proteins in vitro and on the surface of living mammalian cells. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：4990 / 4992

页数：3

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