RNA-Binding Motif Protein 6 is a Candidate Serum Biomarker for Pancreatic Cancer

被引:16
作者
Duan, Baojun [1 ,2 ]
Hu, Xiaoyan [3 ]
Fan, Meiyang [1 ]
Xiong, Xiaofan [1 ]
Han, Lin [1 ]
Wang, Zheng [3 ]
Tong, Dongdong [1 ]
Liu, Liying [1 ]
Wang, Xiaofei [1 ]
Li, Wensheng [4 ]
Yang, Juan [1 ]
Huang, Chen [1 ]
机构
[1] Xi An Jiao Tong Univ, Key Lab Environm & Dis Related Gene, Minist Educ, Dept Cell Biol & Genet,Sch Basic Med Sci,Hlth Sci, Xian 710061, Shaanxi, Peoples R China
[2] Shaanxi Prov Peoples Hosp, Dept Med Oncol, Xian 710068, Shaanxi, Peoples R China
[3] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Xian 710061, Shaanxi, Peoples R China
[4] Shaanxi Prov Peoples Hosp, Dept Pathol, Xian 710068, Shaanxi, Peoples R China
基金
美国国家科学基金会;
关键词
DUCTAL ADENOCARCINOMA; CLINICAL PROTEOMICS; IDENTIFICATION; EXPRESSION; DIAGNOSIS; GENES;
D O I
10.1002/prca.201900048
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Purpose Early diagnosis is crucial to improve outcomes for pancreatic cancer patients (PC). The present study is designed to identify differently expressed peptides involved in PC as potential biomarkers. Experimental Design The serum proteome of 22 PC patients, 12 pancreatitis patients (PP), and 45 healthy controls (HC) are analyzed using magnetic bead-based weak cation exchange (MB-WCX) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Next, a supervised neural network (SNN) algorithm model is established by ClinProTools and the candidate biomarker identified using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Finally, the candidate biomarker is validated in tissue samples. Results The SNN algorithm model discriminates PC from HC with 92.97% sensitivity and 94.55% specificity. Seventy-six differentially expressed peptides are identified, seven of which are significantly different among PC, PP, and HC (p < 0.05). Only one peak (m/z: 1466.99) tends to be upregulated in samples from HC, PP, and PC, which is identified as region of RNA-binding motif protein 6 (RBM6). In subsequent tissue analysis, it is verified that RBM6 expression is significantly higher in PC tissues than paracancerous tissue. Conclusions and Clinical Relevance The results indicate that RBM6 might serve as a candidate diagnostic biomarker for PC. Clinical Relevance Methods used in this study could generate serum peptidome profiles of PC, PP, and HC, and present an approach to identify potential biomarkers for diagnosis of this malignancy.
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页数:5
相关论文
共 25 条
[1]   Strategies for discovering novel pancreatic cancer biomarkers [J].
Chan, Alison ;
Diamandis, Eleftherios P. ;
Blasutig, Ivan M. .
JOURNAL OF PROTEOMICS, 2013, 81 :126-134
[2]   Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients [J].
Duan, Baojun ;
Bai, Jun ;
Qiu, Jian ;
Wang, Jianhua ;
Tong, Cong ;
Wang, Xiaofei ;
Miao, Jiyu ;
Li, Zongfang ;
Li, Wensheng ;
Yang, Juan ;
Huang, Chen .
EBIOMEDICINE, 2018, 37 :134-143
[3]  
Güre AO, 1998, CANCER RES, V58, P1034
[4]   Def-2,-3,-6 and-8, novel mouse genes differentially expressed in the haemopoietic system [J].
Hotfilder, M ;
Baxendale, S ;
Cross, MA ;
Sablitzky, F .
BRITISH JOURNAL OF HAEMATOLOGY, 1999, 106 (02) :335-344
[5]  
Jemal A, 2011, CA-CANCER J CLIN, V61, P134, DOI [10.3322/caac.20115, 10.3322/caac.21492, 10.3322/caac.20107]
[6]  
Jimenez-Luna Cristina, 2018, Oncotarget, V9, P16573, DOI 10.18632/oncotarget.24654
[7]   A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study [J].
Kaur, Sukhwinder ;
Smith, Lynette M. ;
Patel, Asish ;
Menning, Melanie ;
Watley, Duncan C. ;
Malik, Saad S. ;
Krishn, Shiv Ram ;
Mallya, Kavita ;
Aithal, Abhijit ;
Sasson, Aaron R. ;
Johansson, Sonny L. ;
Jain, Maneesh ;
Singh, Shailender ;
Guha, Sushovan ;
Are, Chandrakanth ;
Raimondo, Massimo ;
Hollingsworth, Michael A. ;
Brand, Randall E. ;
Batra, Surinder K. .
AMERICAN JOURNAL OF GASTROENTEROLOGY, 2017, 112 (01) :172-183
[8]  
Kaur S, 2012, BIOMARK MED, V6, P597, DOI [10.2217/BMM.12.69, 10.2217/bmm.12.69]
[9]   Recent advances in proteomic profiling of pancreatic ductal adenocarcinoma and the road ahead [J].
Kim, Hyunsoo ;
Park, Joonho ;
Wang, Joseph I. ;
Kim, Youngsoo .
EXPERT REVIEW OF PROTEOMICS, 2017, 14 (11) :963-971
[10]   Clinical usefulness of carbohydrate antigen 19-9 as a screening test for pancreatic cancer in an asymptomatic population [J].
Kim, JE ;
Lee, KT ;
Lee, JK ;
Paik, SW ;
Rhee, JC ;
Choi, KW .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2004, 19 (02) :182-186