SPOCK1 induces adipose tissue maturation: New insights into the function of SPOCK1 in metabolism

被引：9

作者：

Alshargabi, Rehab ^{[1
]}

Shinjo, Takanori ^{[1
]}

Iwashita, Misaki ^{[1
]}

Yamashita, Akiko ^{[1
]}

Sano, Tomomi ^{[1
]}

Nishimura, Yuki ^{[1
]}

Hayashi, Masato ^{[1
]}

Zeze, Tatsuro ^{[1
]}

Fukuda, Takao ^{[1
]}

Sanui, Terukazu ^{[1
]}

Nishimura, Fusanori ^{[1
]}

机构：

[1] Kyushu Univ, Sect Periodontol, Fac Dent Sci, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 533卷 / 04期

基金：

日本学术振兴会;

关键词：

SPOCK1; Adipocytes; High-fat diet; Adipogenesis; UCP-1; Diabetes; ACTIVATED RECEPTOR-GAMMA; MESENCHYMAL TRANSITION; OBESITY; GENE; EXPRESSION; PERILIPIN; TARGET; WHITE; MAFB;

D O I：

10.1016/j.bbrc.2020.09.129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SPOCK1 is a calcium-binding matricellular proteoglycan that has been extensively studied in several cancer cells. Previously, we generated a mouse line overexpressing SPOCK1 (Spock1-Tg mouse) and showed that SPOCK1 might play an important role in drug-induced gingival overgrowth, indicating that it possesses physiological functions in non-cancer diseases as well. Although SPOCK1 was reported to be secreted from human adipocytes, its role in adipocyte physiology has not been addressed yet. In this study, SPOCK1 protein expression was confirmed in pancreas, adipose tissues, spleen, and liver of normal diet (ND)-fed mice. Interestingly, SPOCK1 was up-regulated in the pancreas and adipose tissues of the high-fat diet (HFD)-fed mice. Spock1-Tg mice fed with ND showed increased maturation in epididymal and inguinal adipose tissues. In addition, Spock1 overexpression strongly decreased expression of UCP-1 in adipose tissues, suggesting that SPOCK1 might regulate thermogenic function through suppression of UCP-1 expression. Finally, exogenous SPOCK1 treatment directly accelerated the differentiation of 3T3-L1 adipocytes, accompanied by the up-regulation of adipocyte differentiation-related gene expression. In conclusion, we demonstrated for the first time that SPOCK1 induced adipocyte differentiation via the upregulation of adipogenesis-related genes. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：1076 / 1082

页数：7

共 34 条

[1] SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth [J].