Isolation of ice-nucleating active bacterium from mackerel and its properties

被引：6

作者：

Chen, ML ^{[1
]}

Chiou, TK ^{[1
]}

Jiang, ST ^{[1
]}

机构：

[1] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 202, Taiwan

来源：

FISHERIES SCIENCE | 2002年 / 68卷 / 04期

关键词：

ice-nucleating activity (INA); number of ice nuclei INA/mL;

D O I：

10.1046/j.1444-2906.2002.00513.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

An ice-nucleating bacterium, designated MACK-4, was isolated from ice-stored mackerel (Scomber australasicus) and identified as a Pseudomonas fluorescens. The optimal temperature and pH for its growth in nutrient broth with 2.5% glycerol (NB-G) were 15degreesC and 6.5, respectively. The maximal ice-nucleating activity (INA) was obtained after 54h incubation at 15degreesC. However, the INA was almost completely lost after 48 h incubation at 25degreesC or higher. The growth and INA decreased with increase of NaCl added in NB-G within 0.0-4.0%. The INA of MACK-4 was very stable at 5-25degreesC, pH 4.0-9.5, while that of isolated ice-nucleating matter from MACK-4 was stable at 5-25degreesC, pH 5.5-9.0.

引用

页码：934 / 941

页数：8

共 29 条

[1] FROST SENSITIVITY OF ZEA-MAYS INCREASED BY APPLICATION OF PSEUDOMONAS-SYRINGAE [J].

ARNY, DC ;

LINDOW, SE ;

UPPER, CD .

NATURE, 1976, 262 (5566) :282-284

[2]

Ashworth E. N., 1992, Horticultural Reviews, V13, P215, DOI 10.1002/9780470650509.ch6

[3] EXPRESSION OF A BACTERIAL ICE NUCLEATION GENE IN PLANTS [J].

BAERTLEIN, DA ;

LINDOW, SE ;

PANOPOULOS, NJ ;

LEE, SP ;

MINDRINOS, MN ;

CHEN, THH .

PLANT PHYSIOLOGY, 1992, 100 (04) :1730-1736

[4] THE SUPERCOOLING OF WATER [J].

BIGG, EK .

PROCEEDINGS OF THE PHYSICAL SOCIETY OF LONDON SECTION B, 1953, 66 (404) :688-694

[5] Man made snow [J].

Brown, R .

SCIENTIFIC AMERICAN, 1997, 276 (01) :119-119

[6] IMMUNOLOGICAL CHARACTERIZATION OF ICE NUCLEATION PROTEINS FROM PSEUDOMONAS-SYRINGAE, PSEUDOMONAS-FLUORESCENS, AND ERWINIA-HERBICOLA [J].

DEININGER, CA ;

MUELLER, GM ;

WOLBER, PK .

JOURNAL OF BACTERIOLOGY, 1988, 170 (02) :669-675

[7]

Franks F., 1985, Biophysics and Biochemistry at Low Temperatures

[8] Ice nucleation in industrial wastewater [J].

Gao, W ;

Smith, DW ;

Sego, DC .

COLD REGIONS SCIENCE AND TECHNOLOGY, 1999, 29 (02) :121-133

[9] Structural basis for the binding of a globular antifreeze protein to ice [J].

Jia, ZC ;

DeLuca, CI ;

Chao, HM ;

Davies, PL .

NATURE, 1996, 384 (6606) :285-288

[10] PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR ICE-NUCLEATING MATTER FROM ERWINIA-UREDOVORA KUIN-3 [J].

KAWAHARA, H ;

MANO, Y ;

OBATA, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (09) :1429-1432

← 1 2 3 →