An improved micropropagation protocol for maritime pine (Pinus pinaster Ait.) isolated cotyledons

被引：25

作者：

Alvarez, J. M. ^{[1
]}

Majada, J. ^{[2
]}

Ordas, R. J. ^{[1
]}

机构：

[1] Univ Oviedo, Area Fisiol Vegetal, Inst Univ Biotecnol, Dept Biol Organismos & Sistemas, E-33071 Oviedo, Spain

[2] Finca Expt La Mata, Serv Reg Invest & Desarrollo Agroalimentario Asti, Secc Forestal, Grado 33820, Asturias, Spain

来源：

FORESTRY | 2009年 / 82卷 / 02期

关键词：

SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; EMBRYOS; COMPETENCE; GROWTH;

D O I：

10.1093/forestry/cpn052

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

In this work an improved micropropagation protocol for Pinus pinaster Ait. using isolated cotyledons is presented. The variables studied during bud induction were embryo preculture, type and concentration of cytokinin and influence of illumination culture conditions. Shoot development and proliferation was obtained by transfer to hormone-free medium with activated charcoal. This protocol also describes a procedure for successful continuous multiplication using axillary shoots and brachyblasts formed during shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be expected per seed after 16 weeks.

引用

页码：175 / 184

页数：10

共 29 条

[1]

Aitken-Christie J., 1988, Genetic manipulation of woody plants., P413

[2]

Alia R., 2003, Technical Guidelines for Genetic Conservation and Use for Maritime Pine (Pinus pinaster Ait.)

[3] An improved micropropagation protocol for stone pine (Pinus pinea L.) [J].