Mutation L232H Promotes Chromophore Maturation of EGFP-Based Fluorescent Fusion Proteins

被引：0

作者：

Simanovskaya, A. A. ^{[1
]}

Ivashina, T. V. ^{[2
]}

Zeifman, Y. S. ^{[1
]}

Fateeva, T. V. ^{[1
]}

Krukova, M. V. ^{[1
]}

Popov, A. N. ^{[3
]}

Kachalova, G. S. ^{[1
,4
]}

Rakitina, T. V. ^{[1
,5
]}

机构：

[1] Natl Res Ctr, Kurchatov Inst, Moscow 123098, Russia

[2] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Region, Russia

[3] European Synchrotron Radiat Facil, F-38000 Grenoble, France

[4] Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia

[5] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

CRYSTALLOGRAPHY REPORTS | 2018年 / 63卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

D O I：

10.1134/S1063774518020256

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The L232H mutant of the enhanced green fluorescent protein (EGFP) was expressed and crystallized. An X-ray diffraction data set was collected from the crystals to 1.53 angstrom resolution. An analysis of the three-dimensional structure revealed a stacking interaction between the amino-acid residues De78 and De232, which contributes to the fastening of the C-terminal region of the protein in the vicinity of the chromophore and influences chromophore maturation of hybrid fluorescent proteins produced by fusion of the target proteins with the C-terminus of EGFP. This hypothesis was experimentally confirmed by investigating chromophore maturation of the hybrid proteins fused to the N- and C-termini of EGFP and EGFP-L232H.

引用

页码：191 / 195

页数：5

共 2 条

[1] Mutation L232H Promotes Chromophore Maturation of EGFP-Based Fluorescent Fusion Proteins
A. A. Simanovskaya
T. V. Ivashina
Y. S. Zeifman
T. V. Fateeva
M. V. Krukova
A. N. Popov
G. S. Kachalova
T. V. Rakitina
Crystallography Reports, 2018, 63 : 191 - 195
[2] A novel method based on recombinant viruses expressing green (EGFP) or red (DsRED) fluorescent proteins to study replicative fitness evolution of nelfinavir-resistant HIV-1 harbouring D30N and L90M mutations in the protease gene
Weber, J
Weberova, J
Kiser, P
Kazanjian, P
Quiñones-Mateu, M
ANTIVIRAL THERAPY, 2004, 9 (04) : U55 - U56

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