Overexpression of MdMIPS1 enhances salt tolerance by improving osmosis, ion balance, and antioxidant activity in transgenic apple

被引：26

作者：

Hu, Lingyu ^{[1
,2
]}

Zhou, Kun ^{[1
]}

Liu, Yuan ^{[1
]}

Yang, Shulin ^{[1
]}

Zhang, Jingyun ^{[1
]}

Gong, Xiaoqing ^{[1
]}

Ma, Fengwang ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Shaanxi Key Lab Apple, Yangling 712100, Shaanxi, Peoples R China

[2] Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Minist Agr,Guangdong Key Lab Trop & Subtrop Fruit, Guangzhou 510640, Peoples R China

来源：

PLANT SCIENCE | 2020年 / 301卷

关键词：

Apple; MdMIPS1; myo-inositol; Salt tolerance; STRESS TOLERANCE; MOLECULAR-MECHANISMS; 1-PHOSPHATE SYNTHASE; MYOINOSITOL; PLANTS; HOMEOSTASIS; EXPRESSION; GENE; AGROBACTERIUM; TRANSCRIPTION;

D O I：

10.1016/j.plantsci.2020.110654

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myo-inositol and its derivatives play vital roles in plant stress tolerance. Myo-inositol-1-phosphate synthase (MIPS) is the rate-limiting enzyme of myo-inositol biosynthesis. However, the role of apple MIPS-mediated myoinositol biosynthesis in stress tolerance remains elusive. In this study, we found that ectopic expression of MdMIPS1 from apple increased myo-inositol content and enhanced tolerance to salt and osmotic stresses in transgenic Arabidopsis lines. In transgenic apple lines over-expressing MdMIPS1, the increased myo-inositol levels could promote accumulation of other osmoprotectants such as glucose, sucrose, galactose, and fructose, to alleviate salinity-induced osmotic stress. Also, it was shown that overexpression of MdMIPS1 enhanced salinity tolerance by improving the antioxidant system to scavenge ROS, as well as Na+ and K+ homeostasis. Taken together, our results revealed a protective role of MdMIPS1-mediated myo-inositol biosynthesis in salt tolerance by improving osmotic balance, antioxidant defense system, and ion homeostasis in apple.

引用

页数：9

共 56 条

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