Fractal analysis of monocytes in diabetes

被引:0
作者
Bianciardi, Giorgio
Tanganelli, Italo
Totagiancaspro, Dorotea
Brogi, Marco
Carducci, Antonietta
De Santi, Maria Margherita
机构
[1] Univ Siena, Dipartimento Pathol Umana & Oncol, Sez Anat & Isotol Patol, I-53100 Siena, Italy
[2] Univ Siena, Sez Diabetol, Dipartimento Sci Odontostomatol, I-53100 Siena, Italy
关键词
fractals; morphometry; monocytes; diabetes;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Fractal dimension of pericellular membrane of monocytes was evaluated in diabetic patients and in control subjects. Monocytes were collected from normal healthy volunteers (n=6) and from diabetic (type 1 and type 2) patients (n=9). Monocytes from healthy volunteers were also stimulated in vitro with the ionophore A23187 or with the oligopeptide FMLP. Monocytes, obtained by Ficoll-Hypaque, were examined with a Philips 300 transmission electron microscope. The cell contour was extracted, resized to a standard dimension and converted to a single pixel outline. Box-counting method was then applied to determine the fractal dimension. Fractal dimensions of monocytes appeared statistically increased in diabetic patients (type 1 and type 2), compared with sex- and age-matched controls (p < 0.01, p < 0.01). The mechanism underlying the observed increased complexity of pericellular membrane may be explained by the in vivo activation of the circulating monocyte in diabetes. In effect, fractal analysis of stimulated in vitro monocytes showed a significant increase of complexity of pericellular membrane, compared with their controls (p < 0.001). Our approach was able to assess and quantitatively evaluate in diabetic patients morphological modifications of the monocyte linked to its activation, offering new parameters useful to follow the effects of therapeutical procedures.
引用
收藏
页码:269 / 272
页数:4
相关论文
共 12 条
[1]   Association of diabetes and hepatitis C infection: Epidemiologic evidence and pathophysiologic insights [J].
Bahtiyar G. ;
Shin J.J. ;
Aytaman A. ;
Sowers J.R. ;
McFarlane S.I. .
Current Diabetes Reports, 2004, 4 (3) :194-198
[2]   Differential diagnosis between mycosis fungoides and chronic dermatitis by fractal analysis [J].
Bianciardi, G ;
Miracco, C ;
De Santi, MM ;
Luzi, P .
JOURNAL OF DERMATOLOGICAL SCIENCE, 2003, 33 (03) :184-186
[3]   Mechanisms of diabetic vasculopathy: An overview [J].
Cooper, ME ;
Bonnet, F ;
Oldfield, M ;
Jandeleit-Dahm, K .
AMERICAN JOURNAL OF HYPERTENSION, 2001, 14 (05) :475-486
[4]   Diabetes and vascular disease -: Pathophysiology, clinical consequences, and medical therapy:: Part I [J].
Creager, MA ;
Lüscher, TF ;
Cosentino, F ;
Beckman, JA .
CIRCULATION, 2003, 108 (12) :1527-1532
[5]   THE FRACTAL DIMENSION MAY BE A USEFUL MORPHOMETRIC DISCRIMINANT IN HISTOPATHOLOGY [J].
CROSS, SS ;
COTTON, DWK .
JOURNAL OF PATHOLOGY, 1992, 166 (04) :409-411
[6]   Oxidative stress and stress-activated signaling pathways: A unifying hypothesis of type 2 diabetes [J].
Evans, JL ;
Goldfine, ID ;
Maddux, BA ;
Grodsky, GM .
ENDOCRINE REVIEWS, 2002, 23 (05) :599-622
[7]   Hyperglycemia and cardiovascular disease in Type 2 diabetes [J].
Laakso, M .
DIABETES, 1999, 48 (05) :937-942
[8]  
Losa GA, 1996, MODERN PATHOL, V9, P174
[9]   Chronic obstructive pulmonary disease, asthma, and risk of type 2 diabetes in women [J].
Rana, JS ;
Mittleman, MA ;
Sheikh, J ;
Hu, FB ;
Manson, JE ;
Colditz, GA ;
Speizer, FE ;
Barr, RG ;
Camargo, CA .
DIABETES CARE, 2004, 27 (10) :2478-2484
[10]   Protection from obesity-induced insulin resistance in mice lacking TNF-alpha function [J].
Uysal, KT ;
Wiesbrock, SM ;
Marino, MW ;
Hotamisligil, GS .
NATURE, 1997, 389 (6651) :610-614