A HIGH PERFORMANCE METHOD FOR THERMODYNAMIC STUDY ON THE BINDING OF COPPER ION AND GLYCINE WITH ALZHEIMER'S AMYLOID β PEPTIDE

被引：16

作者：

Behbehani, G. Rezaei ^{[1
]}

Mirzaie, M. ^{[1
]}

机构：

[1] Imam Khomeini Int Univ, Dept Chem, Qazvin, Iran

来源：

JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY | 2009年 / 96卷 / 02期

基金：

美国国家科学基金会;

关键词：

Alzheimer's amyloid beta peptide; binding parameters; isothermal titration calorimetry; ISOTHERMAL TITRATION CALORIMETRY; AQUEOUS-METHANOL; DENATURATION ENTHALPY; WATER; REPRODUCE; ACETONITRILE; LYSOZYME; ETHANOL; BROMIDE; CALCIUM;

D O I：

10.1007/s10973-008-9311-9

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

The interaction of Cu2+ to the first 16 residues of the Alzheimer's amyloid beta peptide, A beta(1-16) was studied by isothermal titration calorimetry at pH 7.2 and 37 degrees C in aqueous solution. The Gholamreza Rezaei Behbehani (GRB) solvation model was used to reproduce the enthalpies of interactions of A beta(1-16) with glycine, Gly+ A beta(1-16), and Cu2+ ions, Cu2++A beta(1-16), over the whole range of Cu2+ concentrations. The binding parameters recovered from the solvation model were attributed to the structural change of A beta(1-16) due to the glycine and Cu2+ interactions. It was found that there is a set of two identical binding sites for Cu2+ ions. p=2 indicates that the binding has positive cooperativity in the two binding sites. A beta(1-16) structure is destabilized greatly as a result of binding to Cu2+ ions.

引用

页码：631 / 635

页数：5

共 31 条

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Behbehani GR, 2006, B KOREAN CHEM SOC, V27, P208

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