Ultrasensitive Microfluidic Paper-Based Electrochemical Biosensor Based on Molecularly Imprinted Film and Boronate Affinity Sandwich Assay for Glycoprotein Detection

被引:93
作者
Sun, Xiaolu [1 ,2 ]
Jian, Yannan [1 ,2 ]
Wang, He [1 ,2 ]
Ge, Shenguang [1 ]
Yan, Mei [2 ]
Yu, Jinghua [2 ]
机构
[1] Univ Jinan, Inst Adv Interdisciplinary Res, Shandong Collaborat Innovat Ctr Technol & Equipem, Jinan 250022, Shandong, Peoples R China
[2] Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
glycoprotein; molecularly imprinted film; boronate affinity sandwich assay; hybridization chain reaction; electrochemical sensor; STRAND DISPLACEMENT REACTION; DUAL-SIGNAL AMPLIFICATION; PROTEIN GLYCOSYLATION; NANOPARTICLES; RECOGNITION; DNA; NANOSHEETS;
D O I
10.1021/acsami.9b02005
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
In this work, we proposed a strategy that combined molecularly imprinted polymers (MIPs) and hybridization chain reaction into microfluidic paper-based analytical devices for ultrasensitive detection of target glycoprotein ovalbumin (OVA). During the fabrication, Au nanorods with a large surface area and superior conductibility were grown on paper cellulosic fiber as a matrix to introduce a boronate affinity sandwich assay. The composite of MIPs including 4-mercaptophenylboronic acid (MPBA) was able to capture target glycoprotein OVA. SiO(2)pAu nanocomposites labeled MPBA and cerium dioxide (CeO2)-modified nicked DNA double-strand polymers (SiO(2)gAu/dsDNA/CeO2) as a signal tag were captured into the surface of the electrode in the presence of OVA. An electrochemical signal was generated by using nanoceria as redox-active catalytic amplifiers in the presence of 1-naphthol in electrochemical assays. As a result, the electrochemical assay was fabricated and could be applied in the detection of OVA in the wide linear range of 1 pg/mL to 1000 ng/mL with a relatively low detection limit of 0.87 pg/mL (S/N = 3). The results indicated that the proposed platform possessed potential applications in clinical diagnosis and other related fields.
引用
收藏
页码:16198 / 16206
页数:9
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