Characterization of multi-drug resistant Mycobacterium tuberculosis from immigrants residing in the USA using Ion Torrent full-gene sequencing

被引：17

作者：

Daum, L. T. ^{[1
]}

Fischer, G. W. ^{[1
]}

Sromek, J. ^{[2
]}

Khubbar, M. ^{[2
]}

Hunter, P. ^{[2
,3
]}

Gradus, M. S. ^{[2
]}

Bhattacharyya, S. ^{[2
]}

机构：

[1] Longhorn Vaccines & Diagnost, San Antonio, TX USA

[2] City Milwaukee Hlth Dept, Milwaukee, WI USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Madison, WI USA

来源：

EPIDEMIOLOGY AND INFECTION | 2014年 / 142卷 / 06期

关键词：

next-generation sequencing; MDR; Mycobacterium tuberculosis; Drug resistance genes; Ion Torrent sequencing; MUTATIONS; STRAINS; ASSAY;

D O I：

10.1017/S0950268813002409

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Drug-resistant Mycobacterium tuberculosis bacterium (MTB) is spreading worldwide. Three drug-resistant isolates were detected in Burmese, Hmong, and Indian immigrants currently residing in Milwaukee, Wisconsin, USA. Ion Torrent full-gene sequencing and complete genetic analysis was performed within 5 days and compared to results from traditional drug sensitivity testing (DST). Genetic characterization of seven, full-length resistance-associated genes revealed two MDR and one highly resistant strain with important drug-resistant mutations that were confirmed by traditional DST. The rapid turnaround from sample-to-sequence underscores the public health value of Ion Torrent full-gene sequencing of MDR/XDR genes from epidemiologically significant clinical isolates.

引用

页码：1328 / 1333

页数：6

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