Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse

被引：67

作者：

Willig, Katrin I. ^{[1
,2
]}

Steffens, Heinz ^{[1
]}

Gregor, Carola ^{[1
]}

Herholt, Alexander ^{[3
,4
]}

Rossner, Moritz J. ^{[3
,4
]}

Hell, Stefan W. ^{[1
,2
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

[2] Ctr Nanoscale Microscopy & Mol Physiol Brain, Gottingen, Germany

[3] Univ Munich, Dept Mol Neurobiol, Munich, Germany

[4] Univ Munich, Dept Psychiat, D-80539 Munich, Germany

来源：

BIOPHYSICAL JOURNAL | 2014年 / 106卷 / 01期

关键词：

SEMLIKI-FOREST-VIRUS; IN-VITRO; DYNAMICS; MICROSCOPY; VECTORS; BRAIN; VIVO;

D O I：

10.1016/j.bpj.2013.11.1119

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 mu m in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.

引用

页码：L01 / L03

页数：3

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