Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus

被引：5

作者：

Fu, Rongtao ^{[1
]}

Yin, Chuanchun ^{[1
]}

Deng, Qiming ^{[1
]}

Li, Ping ^{[1
]}

Zheng, Aiping ^{[1
]}

机构：

[1] Sichuan Agr Univ, Rice Res Inst, Chengdu, Sichuan, Peoples R China

来源：

TROPICAL PLANT PATHOLOGY | 2014年 / 39卷 / 03期

关键词：

Ustilaginoidea virens; asexual spores; homothallic; MAT; multiplex-PCR; USTILAGINOIDEA-VIRENS; SELF-FERTILITY; SEX;

D O I：

10.1590/S1982-56762014000300003

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Villosiclava virens (anamorph: Ustilaginoidea virens) was isolated from infected rice plants, where it caused false smut. Similar to other ascomycetes, the sexual compatibility of V. virens is controlled by the mating type (MAT) locus. In this study, we applied a PCRbased approach to screen mating type genes in the single asexual spore isolate. Our results showed that V. virens possesses the two master genes required for mating (MAT1-1-1 and MAT1-2-1), suggesting that this fungus is likely to be homothallic. Mating type primer pairs were developed to genotype the single ascospore isolates from different places in China. These analyses provided further evidence that V. virens is a homothallic species and suggest novel genetic mechanisms of sexual reproduction in V. virens.

引用

页码：203 / 209

页数：7

共 31 条

[1] Phylogenetic placement of the anamorphic tribe Ustilaginoideae (Hypocreales, Ascomycota) [J].

Bischoff, JF ;

Sullivan, RF ;

Kjer, KM ;

White, JF .

MYCOLOGIA, 2004, 96 (05) :1088-1094

[2] Fungal Sex and Pathogenesis [J].

Butler, Geraldine .

CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (01) :140-+

[3] Mate recognition in fungi [J].

Casselton, LA .

HEREDITY, 2002, 88 (2) :142-147

[4]

Debuchy R., 2006, MYCOTA GROWTH DIFFER, P293, DOI DOI 10.1007/3-540-28135-5_15

[5] A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis [J].

Dyer, PS ;

Furneaux, PA ;

Douhan, G ;

Murray, TD .

FUNGAL GENETICS AND BIOLOGY, 2001, 33 (03) :173-180

[6] Chromosomal sex-determining regions in animals, plants and fungi [J].

Fraser, JA ;

Heitman, J .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (06) :645-651

[7] Morphological Structure of Propagules and Electrophoretic Karyotype Analysis of False Smut Villosiclava virens in Rice [J].

Fu, Rongtao ;

Ding, Lei ;

Zhu, Jun ;

Li, Ping ;

Zheng, Ai-Ping .

JOURNAL OF MICROBIOLOGY, 2012, 50 (02) :263-269

[8] STRUCTURE AND FUNCTION OF A MATING-TYPE GENE FROM THE HOMOTHALLIC SPECIES NEUROSPORA-AFRICANA [J].

GLASS, NL ;

SMITH, ML .

MOLECULAR AND GENERAL GENETICS, 1994, 244 (04) :401-409

[9] Genetic structure of Mycosphaerella fijiensis populations from Australia, Papua New Guinea and the Pacific Islands [J].

Hayden, HL ;

Carlier, J ;

Aitken, EAB .

PLANT PATHOLOGY, 2003, 52 (06) :703-712

[10] USTILOXINS, ANTIMITOTIC CYCLIC-PEPTIDES FROM FALSE SMUT BALLS ON RICE PANICLES CAUSED BY USTILAGINOIDEA VIRENS [J].

KOISO, Y ;

LI, Y ;

IWASAKI, S ;

HANAOKA, K ;

KOBAYASHI, T ;

SONODA, R ;

FUJITA, Y ;

YAEGASHI, H ;

SATO, Z .

JOURNAL OF ANTIBIOTICS, 1994, 47 (07) :765-773

← 1 2 3 4 →