Efficacy evaluation of the C-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in China

被引：33

作者：

Luo, Yuzi ^{[1
]}

Ji, Shengwei ^{[1
]}

Lei, Jian-Lin ^{[1
]}

Xian, Guang-Tao ^{[1
]}

Liu, Yan ^{[1
]}

Gao, Yao ^{[1
]}

Meng, Xing-Yu ^{[1
]}

Zheng, Guanglai ^{[1
]}

Zhang, En-Yu ^{[1
]}

Wang, Yimin ^{[1
]}

Du, Ming-Liang ^{[1
]}

Li, Yongfeng ^{[1
]}

Li, Su ^{[1
]}

He, Xi-Jun ^{[1
]}

Sun, Yuan ^{[1
]}

Qiu, Hua-Ji ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2017年 / 201卷

基金：

中国国家自然科学基金; 黑龙江省自然科学基金;

关键词：

Classical swine fever virus; Subgenotype; 2.1d; C-strain vaccines; Efficacy; PROTEINS E-RNS; E2; PATHOGENICITY; VIRULENCE;

D O I：

10.1016/j.vetmic.2017.01.012

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Classical swine fever (CSF) is a devastating infectious disease of pigs caused by classical swine fever virus (CSFV). The disease has been controlled following extensive vaccination with the lapinized attenuated vaccine C-strain for decades in China. However, frequent CSF outbreaks occurred recently in a large number of C-strain-vaccinated pig farms in China and a new subgenotype 2.1d of CSFV has been reported to be responsible for the outbreaks. Here we analyzed the molecular variations and antigenic differences among the C-strain-based commercial vaccines of different origins from different manufacturers in China, and reevaluated the vaccines against the emerging subgenotype 2.1 d strain of CSFV. The results showed that the C-strain adapted to the continuous ST cell line (Cyr) contain a unique M290K variation on the E2 protein, compared to those of primary BT cells (CBT) or rabbit origin (CRT) and the traditional C strain sequences available in the GenBank database. Serum neutralization test revealed the antigenic differences between CsT and CBT or CRT. Notably, the neutralizing titers of porcine anti-C-strain sera against the CSFV isolate of subgenotype 2.1d were significantly lower than those against C-strain or Shimen strain. The C-strain-vaccinated, subgenotype 2.1d HLJZZ2014 strain-challenged pigs did not show any clinical signs and all survived. However, these pigs displayed mild pathological and histological lesions, and the CSFV viral RNA was detected in the various tissue and blood samples. Taken together, the C -strain-based vaccines of different origins showed molecular variations and antigenic differences, and could provide clinical but not pathological and virological protection against the subgenotype 2.1d CSFV emerging in China. Further investigation is needed to comprehensively assess the efficacy of C-strain of different doses against the subgenotype 2.1d CSFV. (C)2017 Elsevier B.V. All rights reserved.

引用

页码：154 / 161

页数：8

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