Studying enzymes by in vivo 13C magnetic resonance spectroscopy

A study was conducted to investigate enzymes by in vivo 13C magnetic resonance spectroscopy (C NMR). The theoretical analyses and the experimental methods associated with detecting analyses and the experimental methods were presented. These methods were associated with detecting in vivo 113C magnetization transfer effects of a rapid chemical exchange in vivo 13C magnetization transfer effects of a rapid chemical exchange process between small and large substrate pools. It was demonstrated that the chemical exchange processes of these enzymes was measured by 13C saturation transfer with 13C detection and 13C saturation transfer with 1H detection techniques. It was also found that the exchange between 13C-labeled mitochondrial and cytosolic pools in brain was significantly faster than the tricarboxylic acid TCA) cycle flux.