A highly specific and ultrasensitive two-photon fluorescent probe for imaging native hypochlorous acid in living cells

被引：62

作者：

Zhu, Baocun ^{[1
]}

Wu, Liu ^{[1
]}

Zhu, Hanchuang ^{[1
]}

Wang, Zuokai ^{[1
]}

Duan, Qingxia ^{[1
]}

Fang, Zhaotong ^{[1
]}

Jia, Pan ^{[1
]}

Li, Zilu ^{[1
]}

Liu, Caiyun ^{[1
]}

机构：

[1] Univ Jinan, Sch Resources & Environm, Shandong Prov Engn Technol Res Ctr Ecol Carbon Si, Jinan 250022, Shandong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 269卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Hypochlorous acid (HOCl); Dimethylthiocarbamate (DMTC); Biscoumarin dye; Two-photon fluorescence bioimaging; RECENT PROGRESS; LIVE CELLS; FAR-RED; MITOCHONDRIA; HOCL; BASAL; HCLO; PEROXYNITRITE; MYELOPEROXIDASE; LYSOSOME;

D O I：

10.1016/j.snb.2018.04.115

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monitoring native hypochlorous acid (HOCl) levels in living cells is of great significance because HOCl plays vital roles in various physiological and pathological processes. In this paper, we reported a novel biscoumarin-based two-photon fluorescent probe BCTP for monitoring native HOCl levels in living cells. Probe BCTP containing the recognition group of dimethylthiocarbamate moiety displayed preeminent selectivity towards HOCl over other various bioactive species (1 mM). Additionally, probe BCTP could quantitatively determine HOCl in the range of 0-3 nM with the detection limit of 27 pM. Moreover, probe BCTP could offer a promising tool for the real-time detection of HOCl (response time within 3 s). The potential applications of probe BCTP in the monitoring of native HOCl levels and the fluctuations of endogenous HOCl levels in living cells have been demonstrated by two-photon excitation microscopy with higher sensitivity. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

共 61 条

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