Identification of Differentially Expressed Proteins in Triple-Negative Breast Carcinomas Using DIGE and Mass Spectrometry

被引：48

作者：

Schulz, Daniela M. ^{[1
]}

Boellner, Claudia ^{[1
]}

Thomas, Gerry ^{[2
]}

Atkinson, Mike ^{[3
,4
]}

Esposito, Irene ^{[1
,5
]}

Hoefler, Heinz ^{[1
,5
]}

Aubele, Michaela ^{[1
,5
]}

机构：

[1] Helmholtz Zentrum Munchen, Inst Pathol, German Res Ctr Environm Hlth, D-85764 Neuherberg, Germany

[2] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, London W12 0NN, England

[3] Helmholtz Zentrum Munchen, Inst Radiat Biol, German Res Ctr Environm Hlth, D-85764 Neuherberg, Germany

[4] Tech Univ Munich, Klin & Poliklin Strahlentherapie, Klinikum Rechts Isar, D-81675 Munich, Germany

[5] Tech Univ Munich, Inst Pathol, D-81675 Munich, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 07期

关键词：

proteomics; breast cancer; triple-negative; 2D-DIGE; MALDI-TOF/TOFMS; ANNEXIN A1 EXPRESSION; OXIDATIVE STRESS; MESSENGER-RNA; CANCER CELLS; LACTOFERRIN; RECEPTOR; GENE; PROTEOMICS; PHENOTYPE; MARKERS;

D O I：

10.1021/pr900071h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the protein expression pattern of triple-negative breast carcinomas (HER2-, ER-, PR-) versus those being positive for HER2 and negative for the hormone receptors (HER2+, ER-, PR-) by 2-D DIGE and mass spectrometry. We obtained differential expression patterns for several glycolytic enzymes (as for example MDH2, PGK1, TKT, Aldolase1), cytokeratins (CK7, 8, 9, 14, 17, 19), further structure proteins (vimentin, fibronectin, L-plastin), for NME1-NME2, lactoferrin, and members of the Annexin family. Western blot analysis and immunohistochemistry were conducted to verify the results. The identified marker proteins may advance a more detailed characterization of triple-negative breast cancers and may contribute to the development of better treatment strategies.

引用

页码：3430 / 3438

页数：9

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